Fourier Transform Infrared Microspectroscopy Combined with Principal Component Analysis and Artificial Neural Networks for the Study of the Effect of β-Hydroxy-β-Methylbutyrate (HMB) Supplementation on Articular Cartilage
| Autor: | Katarzyna Kras, Hauke Clausen-Schaumann, Marcin Bartłomiej Arciszewski, Marta Arczewska, Michał Świetlicki, Ewa Tomaszewska, Tomasz Blicharski, Mariusz Gagoś, Izabela Świetlicka, Siemowit Muszyński, Attila Aszodi, Carina Prein, Piotr Dobrowolski |
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| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular Male collagen QH301-705.5 Swine Articular cartilage Catalysis Article β-hydroxy-β-methylbutyrate supplementation Inorganic Chemistry symbols.namesake Elastic Modulus Spectroscopy Fourier Transform Infrared Valerates Animals articular cartilage Biology (General) Physical and Theoretical Chemistry Fourier transform infrared spectroscopy skin and connective tissue diseases FTIR microspectroscopy QD1-999 Molecular Biology Spectroscopy Principal Component Analysis atomic force microscopy biology Atomic force microscopy Chemistry animal model Organic Chemistry Histology General Medicine Computer Science Applications Fourier transform Proteoglycan Principal component analysis Dietary Supplements biology.protein symbols proteoglycans Neural Networks Computer Tissue stiffness artificial neural networks Biomedical engineering |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 17 International Journal of Molecular Sciences, Vol 22, Iss 9189, p 9189 (2021) |
| ISSN: | 1422-0067 |
| Popis: | The potential of Fourier Transform infrared microspectroscopy (FTIR microspectroscopy) and multivariate analyses were applied for the classification of the frequency ranges responsible for the distribution changes of the main components of articular cartilage (AC) that occur during dietary β-hydroxy-β-methyl butyrate (HMB) supplementation. The FTIR imaging analysis of histological AC sections originating from 35-day old male piglets showed the change in the collagen and proteoglycan contents of the HMB-supplemented group compared to the control. The relative amount of collagen content in the superficial zone increased by more than 23% and in the middle zone by about 17%, while no changes in the deep zone were observed compared to the control group. Considering proteoglycans content, a significant increase was registered in the middle and deep zones, respectively 62% and 52% compared to the control. AFM nanoindentation measurements collected from animals administered with HMB displayed an increase in AC tissue stiffness by detecting a higher value of Young’s modulus in all investigated AC zones. We demonstrated that principal component analysis and artificial neural networks could be trained with spectral information to distinguish AC histological sections and the group under study accurately. This work may support the use and effectiveness of FTIR imaging combined with multivariate analyses as a quantitative alternative to traditional collagenous tissue-related histology. |
| Databáze: | OpenAIRE |
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