ROC analysis of p16 expression in cell blocks of metastatic head and neck squamous cell carcinoma
| Autor: | Mark G. Ettel, Abberly A. Lott Limbach, Anna-Karoline Israel, Bennett L. Wilson |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biopsy Fine-Needle 030209 endocrinology & metabolism Alphapapillomavirus Sensitivity and Specificity Stain Pathology and Forensic Medicine Metastasis 03 medical and health sciences 0302 clinical medicine Biomarkers Tumor Carcinoma Humans Medicine Cyclin-Dependent Kinase Inhibitor p16 In Situ Hybridization Receiver operating characteristic medicine.diagnostic_test Squamous Cell Carcinoma of Head and Neck business.industry Papillomavirus Infections Middle Aged medicine.disease Immunohistochemistry Head and neck squamous-cell carcinoma Oropharyngeal Neoplasms medicine.anatomical_structure Fine-needle aspiration ROC Curve Cervical lymph nodes Lymphatic Metastasis 030220 oncology & carcinogenesis RNA Viral Female Lymph Nodes business Neck |
| Zdroj: | Journal of the American Society of Cytopathology. 10:423-428 |
| ISSN: | 2213-2945 |
| DOI: | 10.1016/j.jasc.2021.03.004 |
| Popis: | Background Oropharyngeal squamous cell carcinoma is associated with human papillomavirus (HPV) and often presents with early metastasis to cervical neck lymph nodes that are amenable to fine-needle aspiration (FNA). The most common method of HPV status determination is p16 immunohistochemistry (IHC). The literature suggests that a lower threshold is needed for p16 positivity on cell block. We examined and quantified p16 IHC staining on cell block and used receiver operating characteristics (ROC) curve analysis to determine an optimal cutoff value with high sensitivity and specificity. Methods Thirty-six FNAs of metastatic squamous cell carcinoma from cervical lymph nodes with p16 IHC were evaluated. The p16 stain was quantified in 5% increments and high-risk HPV mRNA in situ hybridization was performed as a gold standard test. Statistical analysis was performed. Results Interobserver variability was evaluated and was shown to be low with an intraclass correlation coefficient of 0.857. ROC analysis was performed and showed that a cell block p16 IHC cutoff of 15% yielded the highest sensitivity (80%) and specificity (81.8%). Conclusion Our data show that a threshold of 15% p16 staining in cell block maximizes sensitivity and specificity. |
| Databáze: | OpenAIRE |
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