Synthesis, characterization, and assessment of cytotoxic, antiproliferative, and antiangiogenic effects of a novel procainamide hydrochloride-poly(maleic anhydride-co-styrene) conjugate
| Autor: | Ali Fazil Yenidunya, Zubeyde Akin Polat, Gulderen Karakus, Mesut Karahan, Ayse Sahin Yaglioglu |
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| Přispěvatelé: | Üsküdar Üniversitesi, Mühendislik Fakültesi, Biyomühendislik, TR103146, [Karakus, Gulderen] Cumhuriyet Univ, Sch Med, CUTFAM RCCUSM, Res Ctr, TR-58140 Sivas, Turkey -- [Polat, Zubeyde Akin] Cumhuriyet Univ, Sch Med, Dept Parasitol, TR-58140 Sivas, Turkey -- [Yaglioglu, Ayse Sahin] Cankiri Karatekin Univ, Dept Chem, Fac Sci, TR-18100 Cankiri, Turkey -- [Karahan, Mesut] Uskudar Univ, Fac Engn & Nat Sci, Dept Bioengn, TR-34662 Uskudar Istanbul, Turkey -- [Yenidunya, Ali Fazil] Cumhuriyet Univ, Fac Sci, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey, KARAKUS, Gulderen -- 0000-0003-2596-9208, Yenidunya, Ali Fazil -- 0000-0002-9886-977X, Karahan, Mesut -- 0000-0002-8971-678X |
| Rok vydání: | 2012 |
| Předmět: |
antiproliferative activity
Polymers Biomedical Engineering Biophysics Angiogenesis Inhibitors Bioengineering Procainamide poly(maleic anhydride-co-styrene) modification Cell Line Biomaterials HeLa Mice chemistry.chemical_compound Animals Cytotoxicity Cell Proliferation Maleic Anhydrides Procainamide Hydrochloride procainamide hydrochloride biology Cell growth Maleic anhydride Nuclear magnetic resonance spectroscopy biology.organism_classification Biochemistry chemistry Cell culture cytotoxicity antiangiogenic effects Nuclear chemistry Conjugate |
| Zdroj: | Journal of Biomaterials Science, Polymer Edition. 24:1260-1276 |
| ISSN: | 1568-5624 0920-5063 |
| Popis: | WOS: 000319496400007 PubMed ID: 23713427 Poly(maleic anhydride-co-styrene) (MAST) was synthesized by a free-radical polymerization reaction. A bioactive molecule, procainamide hydrochloride (PH), was then conjugated to MAST. The conjugation product was named as MAST/PH. Structural characterization of MAST and MAST/PH was carried out by Fourier Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Their molecular weights were determined by size-exclusion chromatography. A mechanism was then suggested for the conjugation reaction. The results of the cytotoxicity assay, employing a mouse fibroblast cell line (L929), indicated that MAST/PH had no cytotoxicity at concentrations 62gmL(1) (p>0.05). Antiproliferative activities of MAST/PH and PH were determined by the BrdU cell proliferation ELISA assay, using C6 and HeLa cell lines. In the experiment, two anticancer chemotherapy drugs, cisplatin and 5-fluorouracil, were included as positive control. Antiproliferative activity results demonstrated that MAST/PH yielded the highest suppression profile (approximately 42%) at 20g/ml, while free PH exerted the same activity at 100g/ml. Interestingly, both MAST/PH and PH suppressed the proliferation of only one of the cell lines, C6 cells. Both cisplatin and 5-fluorouracil yielded approximately 60% antiproliferative activity on C6 cells at 20 and 100g/ml concentrations. Antiangiogenic capacity of both MAST and MAST/PH was also investigated by using the chicken chorioallantoic membrane assay. Results obtained indicated that while MAST/PH could be included into the category of good antiangiogenic substances, the activity score of MAST was within the weak category. Sciences Research Projects Foundation of Cumhuriyet University [F258] This work was supported by Sciences Research Projects Foundation of Cumhuriyet University (Project No: F258). The structural characterizations were carried out at Technology Research and Developing Centre, Erciyes University, Kayseri, Turkey. |
| Databáze: | OpenAIRE |
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