On-Chip Stretching, Sorting, and Electro-Optical Nanopore Sensing of Ultralong Human Genomic DNA
| Autor: | Diana Huttner, Adam Zrehen, Amit Meller |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
single-molecule sensing
genomic DNA Materials science Optical Phenomena Microfluidics microfluidics General Physics and Astronomy Nanofluidics 02 engineering and technology nanofluidics 010402 general chemistry 01 natural sciences Fluorescence Article Nanopores chemistry.chemical_compound Electricity Humans electro-optical sensing General Materials Science Fluidics Nick translation Electrodes Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Genome Human business.industry Lasers Biomolecule General Engineering DNA HCT116 Cells 021001 nanoscience & nanotechnology solid-state nanopore 0104 chemical sciences Nanopore chemistry Optoelectronics 0210 nano-technology business |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/acsnano.9b07873 |
| Popis: | Solid-state nanopore sensing of ultralong genomic DNA molecules has remained challenging, as the DNA must be controllably delivered by its leading end for efficient entry into the nanopore. Herein, we introduce a nanopore sensor device designed for electro-optical detection and sorting of ultralong (300+ kilobase pair) genomic DNA. The fluidic device, fabricated in-silicon and anodically bonded to glass, uses pressure-induced flow and an embedded pillar array for controllable DNA stretching and delivery. Extremely low concentrations (50 fM) and sample volumes (∼1 μL) of DNA can be processed. The low height profile of the device permits high numerical aperture, high magnification imaging of DNA molecules, which remain in focus over extended distances. We demonstrate selective DNA sorting based on sequence-specific nick translation labeling and imaging at high camera frame rates. Nanopores are fabricated directly in the assembled device by laser etching. We show that uncoiling and stretching of the ultralong DNA molecules permits efficient nanopore capture and threading, which is simultaneously and synchronously imaged and electrically measured. Furthermore, our technique provides key insights into the translocation behavior of ultralong DNA and promotes the development of all-in-one micro/nanofluidic platforms for nanopore sensing of biomolecules. |
| Databáze: | OpenAIRE |
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