Turkey and chicken interferon-ϒ, which share high sequence identity, are biologically cross-reactive

Autor: Peter K. Kaiser, K. Venugopal, Ken Howes, Shelly Lawson, Lisa Rothwell, Benedicte Lambrecht
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Zdroj: Lawson, S, Rothwell, L, Lambrecht, B, Howes, K, Venogopal, K & Kaiser, P 2001, ' Turkey and chicken interferon-ϒ, which share high sequence identity, are biologically cross-reactive ', Developmental and Comparative Immunology, vol. 25, no. 1, pp. 69-82 . https://doi.org/10.1016/S0145-305X(00)00044-6
DOI: 10.1016/S0145-305X(00)00044-6
Popis: Turkey and chicken interferon-gamma (IFN-gamma) share high identity (96.3% and 97% at the nucleotide and amino acid level, respectively). As such, we predicted that they would be functionally cross-reactive. To test this hypothesis, we produced recombinant turkey and chicken IFN-gamma, and compared their biological properties. Recombinant turkey and chicken IFN-gamma both induce HD11 cells (a chicken macrophage cell line) and LSTC-IAH30 cells (ALV-J-transformed turkey macrophages) to produce nitric oxide (NO), as measured in an avian IFN-gamma bioassay. Polyclonal and monoclonal antibodies, capable of neutralising the effect of chicken IFN-gamma on HD11 cells, were also shown to inhibit the activity of turkey IFN-gamma on these cells. The antibody neutralisation effect on both turkey and chicken IFN-gamma was shown by a significant reduction in NO production by HD11 cells when the neutralising antibodies were present in the bioassay. FACS analysis showed that HD11 and LSTC-IAH30 cells share some cell surface markers.
Databáze: OpenAIRE
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