Turkey and chicken interferon-ϒ, which share high sequence identity, are biologically cross-reactive
Autor: | Peter K. Kaiser, K. Venugopal, Ken Howes, Shelly Lawson, Lisa Rothwell, Benedicte Lambrecht |
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Jazyk: | angličtina |
Rok vydání: | 2001 |
Předmět: |
Chickens/metabolism
Turkeys animal structures medicine.drug_class Immunology Sequence Homology Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody Neutralization law.invention Interferon-gamma law medicine Bioassay Animals Interferon gamma Cells Cultured COS cells biology Sequence Homology Amino Acid Macrophages Flow Cytometry Molecular biology Recombinant Proteins Polyclonal antibodies COS Cells Recombinant DNA biology.protein Antibody Chickens Developmental Biology medicine.drug |
Zdroj: | Lawson, S, Rothwell, L, Lambrecht, B, Howes, K, Venogopal, K & Kaiser, P 2001, ' Turkey and chicken interferon-ϒ, which share high sequence identity, are biologically cross-reactive ', Developmental and Comparative Immunology, vol. 25, no. 1, pp. 69-82 . https://doi.org/10.1016/S0145-305X(00)00044-6 |
DOI: | 10.1016/S0145-305X(00)00044-6 |
Popis: | Turkey and chicken interferon-gamma (IFN-gamma) share high identity (96.3% and 97% at the nucleotide and amino acid level, respectively). As such, we predicted that they would be functionally cross-reactive. To test this hypothesis, we produced recombinant turkey and chicken IFN-gamma, and compared their biological properties. Recombinant turkey and chicken IFN-gamma both induce HD11 cells (a chicken macrophage cell line) and LSTC-IAH30 cells (ALV-J-transformed turkey macrophages) to produce nitric oxide (NO), as measured in an avian IFN-gamma bioassay. Polyclonal and monoclonal antibodies, capable of neutralising the effect of chicken IFN-gamma on HD11 cells, were also shown to inhibit the activity of turkey IFN-gamma on these cells. The antibody neutralisation effect on both turkey and chicken IFN-gamma was shown by a significant reduction in NO production by HD11 cells when the neutralising antibodies were present in the bioassay. FACS analysis showed that HD11 and LSTC-IAH30 cells share some cell surface markers. |
Databáze: | OpenAIRE |
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