Acquired Resistance to Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancers: The Role of Next-Generation Sequencing on Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Samples
Autor: | Filippo de Marinis, Valeria Midolo De Luca, Massimo Barberis, Antonio Passaro, Davide Vacirca, Chiara Casadio, Elena Guerini-Rocco, Juliana Guarize |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Lung Neoplasms DNA sequencing Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Acquired resistance Carcinoma Non-Small-Cell Lung medicine Biomarkers Tumor Humans Endobronchial ultrasound Endoscopic Ultrasound-Guided Fine Needle Aspiration Protein Kinase Inhibitors Lung Molecular Diagnostic Testing business.industry High-Throughput Nucleotide Sequencing General Medicine Protein-Tyrosine Kinases Medical Laboratory Technology 030104 developmental biology medicine.anatomical_structure Cytopathology Drug Resistance Neoplasm 030220 oncology & carcinogenesis Female Non small cell business Tyrosine kinase |
Zdroj: | Archives of pathologylaboratory medicine. 142(4) |
ISSN: | 1543-2165 |
Popis: | Context.— Molecular testing is essential for the diagnostic workup of patients with advanced non–small cell lung cancers. Cytology specimens from minimally invasive procedures, such as endobronchial ultrasound–guided transbronchial needle aspiration, are often the only available samples for these patients. The implementation of molecular diagnostic testing, and in particular next-generation sequencing–based testing, on these cytologic specimens is currently an evolving field for lung cytopathology. The application of these molecular analyses on tyrosine kinase inhibitor–resistant non–small cell lung cancers raises unique technical, biologic, and clinical challenges. Objective.— To provide an overview of the implementation of next-generation sequencing analysis on endobronchial ultrasound–guided transbronchial needle aspiration samples to detect the molecular aberrations underneath the phenomenon of acquired resistance in patients with non–small cell lung cancers progressing while on the EGFR/ALK tyrosine kinase inhibitor treatment. Data Sources.— Peer-reviewed original articles, review articles, and published guidelines and expert opinion reports were reviewed, together with our single-center experience. Conclusions.— Next-generation sequencing analyses and the endobronchial ultrasound–guided transbronchial needle aspiration procedure may represent a valuable strategy to address the unique requirements of molecular testing on tyrosine kinase inhibitor–resistant non–small cell lung cancers. |
Databáze: | OpenAIRE |
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