Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src
| Autor: | Hiroyuki Nunoi, Karen J. Lomax, William M. Nauseef, Joan M.G. Sechler, John I. Gallin, Robert A. Clark, Bryan D. Volpp, Harry L. Malech, Thomas L. Leto |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Neutrophils
Molecular Sequence Data Proto-Oncogene Proteins pp60(c-src) Molecular cloning Biology Granulomatous Disease Chronic chemistry.chemical_compound Complementary DNA Proto-Oncogene Proteins Sequence Homology Nucleic Acid Animals Humans NADH NADPH Oxidoreductases Amino Acid Sequence Cloning Molecular Oxidase test Multidisciplinary Base Sequence Superoxide NADPH Oxidases Protein-Tyrosine Kinases Molecular biology chemistry Biochemistry Sequence motif Tyrosine kinase Nicotinamide adenine dinucleotide phosphate Proto-oncogene tyrosine-protein kinase Src |
| Zdroj: | Science (New York, N.Y.). 248(4956) |
| ISSN: | 0036-8075 |
| Popis: | Chronic granulomatous diseases (CGDs) are characterized by recurrent infections resulting from impaired superoxide production by a phagocytic cell, nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) oxidase. Complementary DNAs were cloned that encode the 67-kilodalton (kD) cytosolic oxidase factor (p67), which is deficient in 5% of CGD patients. Recombinant p67 (r-p67) partially restored NADPH oxidase activity to p67-deficient neutrophil cytosol from these patients. The p67 cDNA encodes a 526-amino acid protein with acidic middle and carboxyl-terminal domains that are similar to a sequence motif found in the noncatalytic domain of src-related tyrosine kinases. This motif was recently noted in phospholipase C-gamma, nonerythroid alpha-spectrin (fodrin), p21ras-guanosine triphophatase-activating protein (GAP), myosin-1 isoforms, yeast proteins cdc-25 and fus-1, and the 47-kD phagocyte oxidase factor (p47), which suggests the possibility of common regulatory features. |
| Databáze: | OpenAIRE |
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