ABCE1 Is a Highly Conserved RNA Silencing Suppressor
| Autor: | Erkki Truve, Peter Swoboda, Alla Piirsoo, Karl Ekwall, Per Kylsten, Agata Smialowska, Lenne Nigul, Kadri Vinkel, Jelena Gerassimenko, Kairi Kärblane, Cecilia Sarmiento |
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| Rok vydání: | 2015 |
| Předmět: |
RNA-induced transcriptional silencing
RNA-induced silencing complex lcsh:Medicine Biology 03 medical and health sciences RNA interference Tobacco Animals Humans RasiRNA Signal recognition particle RNA Caenorhabditis elegans Caenorhabditis elegans Proteins lcsh:Science Plant Proteins 030304 developmental biology Genetics 0303 health sciences Multidisciplinary lcsh:R 030302 biochemistry & molecular biology Peptide Chain Termination Translational Non-coding RNA RNA silencing HEK293 Cells RNA editing ATP-Binding Cassette Transporters RNA Interference lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 2, p e0116702 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0116702 |
| Popis: | ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosome-recycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference. |
| Databáze: | OpenAIRE |
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