Sequential activation of p75 and TrkB is involved in dendritic development of subventricular zone-derived neuronal progenitorsin vitro

Autor: Eduardo Gascon, M. J. Barral-Moran, Laszlo Vutskits, Huanxiang Zhang, P. J. Kiss, Christophe Mas, Jozsef Zoltan Kiss
Rok vydání: 2005
Předmět:
Sialic Acids/metabolism
Time Factors
Gamma-Aminobutyric Acid/metabolism
Polymerase Chain Reaction/methods
Neural Cell Adhesion Molecule L1/metabolism
Nerve Tissue Proteins/metabolism
Tropomyosin receptor kinase B
Neurotrophin 3/pharmacology
Tropomyosin receptor kinase A
Polymerase Chain Reaction
Receptor
Nerve Growth Factor
Tropomyosin receptor kinase C
Cerebral Ventricles
Nestin
Rats
Sprague-Dawley
Diagnostic Imaging/methods
GAP-43 Protein
Intermediate Filament Proteins
Neurotrophin 3
Tubulin
Neurotrophic factors
Nerve Growth Factor
Immunohistochemistry/methods
Low-affinity nerve growth factor receptor
Dendrites/drug effects/ physiology
Stem Cells/ physiology
Receptor
trkB/ metabolism
gamma-Aminobutyric Acid
Cells
Cultured
Neurons
GAP-43 Protein/metabolism
Cell Death
Stem Cells
General Neuroscience
Neurons/drug effects/ physiology
Immunohistochemistry
medicine.anatomical_structure
Actins/metabolism
Receptors
Nerve Growth Factor/ metabolism
Microtubule-Associated Proteins/metabolism
Microtubule-Associated Proteins
Proto-Oncogene Proteins c-fos
Neurotrophin
Diagnostic Imaging
Cerebral Ventricles/ cytology
DNA
Complementary
animal structures
Receptor
trkA/metabolism
Intermediate Filament Proteins/metabolism
Subventricular zone
Nerve Tissue Proteins
Neural Cell Adhesion Molecule L1
Brain-Derived Neurotrophic Factor/pharmacology
Receptors
Nerve Growth Factor
Biology
In Situ Nick-End Labeling/methods
In Situ Nick-End Labeling
medicine
Receptor
trkB
Animals
Receptor
trkA
Brain-Derived Neurotrophic Factor
Proto-Oncogene Proteins c-fos/metabolism
Dendrites
Tubulin/metabolism
Actins
ddc:616.8
Rats
Animals
Newborn
Nerve Growth Factor/pharmacology
nervous system
DNA
Complementary/biosynthesis
Trk receptor
Sialic Acids
biology.protein
Neuroscience
Zdroj: European Journal of Neuroscience, Vol. 21, No 1 (2005) pp. 69-80
ISSN: 1460-9568
0953-816X
DOI: 10.1111/j.1460-9568.2004.03849.x
Popis: Dendritic arbor development of subventricular zone-derived interneurons is a critical step in their integration into functional circuits of the postnatal olfactory bulb. However, the mechanism and molecular control of this process remain unknown. In this study, we have developed a culture model where dendritic development of purified subventricular zone cells proceeds under serum-free conditions in the absence of added growth factors and non-neural cells. We demonstrate that the large majority of these cells in culture express GABA and elaborate dendritic arbors with spine-like protrusions but they do not possess axons. These neurons expressed receptors for neurotrophins including p75, TrkB and TrkC but not TrkA. Application of exogenous neurotrophins, including brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and nerve growth factor (NGF), to cultures stimulated dendritic growth and led to more complex dendritic arbors during the initial 3 days in culture. Our results suggest that these effects are independent of Trk receptors and mediated by the p75/ceramide signaling pathway. We also show that brain-derived neurotrophic factor is the only neurotrophin that is able to influence late-phase dendritic development via TrkB receptor activation. These results suggest that dendritic arbor development of subventricular zone-derived cells may be regulated by neurotrophins through the activation of p75 and the TrkB receptor signaling pathways in a sequentially defined temporal pattern.
Databáze: OpenAIRE
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