Conformational transitions of antibodies induced by hapten binding
| Autor: | Vladimir P. Zav'yalov, G.V. Troitsky, Vyacheslav M. Abramov, V.G. Skvortzov |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Globulin
Protein Conformation chemical and pharmacologic phenomena Biochemistry Genetics and Molecular Biology (miscellaneous) Cell Line Native state Molecule Animals Humans Tyrosine Transition (genetics) biology Effector Chemistry Temperature Kinetics Myeloma Proteins Biochemistry Immunoglobulin G biology.protein Biophysics Spectrophotometry Ultraviolet Binding Sites Antibody Rabbits Antibody Hapten Haptens |
| Zdroj: | Biochimica et biophysica acta. 493(2) |
| ISSN: | 0006-3002 |
| Popis: | The conformational changes of antibody structure induced by hapten molecule binding were investigated by means of thermal perturbation difference spectroscopy. The studies of the free rabit anti-dinitrophenyl antibodies show the conformational transition at temperatures between 25 and 35 degrees C. The changes occurring at the higher temperature are accompanied by the screening of the significant part of exposed tyrosine residues. Binding of the hapten molecules induces a similar transition to that which occurs between the two temperature dependent states of the free antibody. In contrast to our previous results with anti-dansyl rabbit antibodies the dinitrophenyl lysine stabilizes the "low temperature" native state of the protein. The investigation of the MOPC-315 mouse immunoglobulin A myeloma protein possessing anti-dinitrophenyl activity indicates no conformational transition at temperatures between 25 and 35 degrees C and only a small decrease of tyrosine exposure induced by the hapten binding. Our present and previous results indicate that most of the free immunoglobulins exist in two native conformational states which have a small difference in free energy. Hapten binding causes the transition in equilibrium between the two states towards the one of better binding. It is possible that this transition is necessary but not sufficient step for inducing the effector function of antibodies. |
| Databáze: | OpenAIRE |
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