Replacement Process of Carbonate Apatite by Alveolar Bone in a Rat Extraction Socket
| Autor: | Jiong Yan Gu, Xiaoxu Zhang, Yasunori Ayukawa, Jing Qi Zhang, Ryosuke Takahashi, Ikue Narimatsu, Nobuyuki Ueda, Kiyoshi Koyano, Ikiru Atsuta, Yuki Egashira |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
autogenous bone Cell morphology Article Mandibular second molar In vivo Osteoclast Precursor cell medicine carbonate apatite General Materials Science Dental alveolus Microscopy QC120-168.85 biology Chemistry QH201-278.5 Acid phosphatase bone substitute Engineering (General). Civil engineering (General) In vitro TK1-9971 medicine.anatomical_structure Descriptive and experimental mechanics osteoclast biology.protein Electrical engineering. Electronics. Nuclear engineering TA1-2040 Biomedical engineering |
| Zdroj: | Materials, Vol 14, Iss 4457, p 4457 (2021) Materials Volume 14 Issue 16 |
| ISSN: | 1996-1944 |
| Popis: | The objective of this study was to investigate a bone graft substitute containing carbonate apatite (CO3Ap) to analyze bone replacement and the state of bone formation in vitro and in vivo compared with autogenous bone (AB) or control. An osteoclast precursor cell line was cultured with AB or CO3Ap, and morphological analysis using scanning electron microscopy and a tartrate-resistant acid phosphatase activity assay were performed. The right maxillary first and second molars of Wistar rats were extracted and compensated by AB or CO3Ap granules. Following implantation, the bone formation state was evaluated after 3, 5, 7, 14, and 28 days of surgery by micro-computed tomography and immunohistostaining. The osteoclast-like cell morphology was typical with many cell protrusions in the AB and CO3Ap groups. Additionally, the number of osteoclast-like cells formed in the culture increased in each group however, there was no significant difference between the AB and CO3Ap groups. Five days after tooth extraction, osteoclasts were observed near CO3Ap. The bone thickness in the CO3Ap group was significantly increased than that in the control group and the bone formation in the CO3Ap group increased by the same level as that in the AB group. CO3Ap is gradually absorbed by osteoclasts in the extraction socket and is easily replaced by alveolar bone. The process of bone replacement by osteoclasts is similar to that of autologous bone. By observing the process of bone replacement in more detail, it may be possible to gain a better understanding of the bone formation and control the amount of bone after surgery. |
| Databáze: | OpenAIRE |
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