BMP signaling-driven osteogenesis is critically dependent on Prdx-1 expression-mediated maintenance of chondrocyte prehypetrophy
| Autor: | Amitabha Bandyopadhyay, Yogesh Kumar, Naibedya Chattopadhyay, Sandeep Kumar, Arun Kumar Trivedi, Anukampa Shukla, Sabyasachi Sanyal, Gatha Thacker, Jitendra Kumar Kanaujiya, Tathagata Biswas, Kainat Khan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cellular differentiation Chick Embryo Cell Enlargement Peroxiredoxin 1 Bone morphogenetic protein Biochemistry Chondrocyte Mice 03 medical and health sciences Chondrocytes Osteogenesis Physiology (medical) medicine Animals BMP signaling pathway Endochondral ossification Chemistry Cartilage Cell Differentiation Peroxiredoxins Rats Cell biology 030104 developmental biology medicine.anatomical_structure Bone Morphogenetic Proteins Signal transduction Signal Transduction |
| Zdroj: | Free Radical Biology and Medicine. 118:1-12 |
| ISSN: | 0891-5849 |
| Popis: | During endochondral ossification, cartilage template is eventually replaced by bone. This process involves several well characterized, stereotypic, molecular and cellular changes in the cartilage primordia. These steps involve transition from resting to proliferative and then pre-hypertrophic to finally hypertrophic cartilage. BMP signaling is necessary and sufficient for osteogenesis. However, the specific step(s) of endochondral ossification in which BMP signaling plays an essential role is not yet known. In this study we have identified Prdx1, a known scavenger of ROS, to be expressed in pre-hypertrophic chondrocytes in a BMP signaling-dependent manner. We demonstrate that BMP signaling inhibition increases ROS levels in osteogenic cells. Further, Prdx1 regulates osteogenesis in vivo by helping maintenance of Ihh expressing pre-hypertrophic cells, in turn regulating these cells' transition into hypertrophy. Therefore, our data suggests that one of the key roles of BMP signaling in endochondral ossification is to maintain pre-hypertrophic state. |
| Databáze: | OpenAIRE |
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