Understanding the Underlying Molecular Mechanisms of Meiotic Arrest during In Vitro Spermatogenesis in Rat Prepubertal Testicular Tissue
Autor: | Justine Saulnier, Frédéric Chalmel, Marion Delessard, Laura Moutard, Tony Pereira, François Fraissinet, Ludovic Dumont, Aurélie Rives-Feraille, Christine Rondanino, Nathalie Rives |
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Přispěvatelé: | Chard-Hutchinson, Xavier, Neuroendocrine, Endocrine and Germinal Differentiation Communication (NorDic), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), CHU Rouen, Normandie Université (NU), Rouen Normandy University, Ligue contre le Cancer-Comite de Seine-Maritime, Fondation de l'Avenir [AP-RM-18-031], UNIROUEN - UFR Santé (UNIROUEN UFR Santé), Normandie Université (NU)-Normandie Université (NU) |
Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
Male
steroidogenesis [SDV.BDD.GAM] Life Sciences [q-bio]/Development Biology/Gametogenesis Organic Chemistry RNA-sequencing rat prepubertal testis General Medicine in vitro spermatogenesis Catalysis Rats Computer Science Applications Inorganic Chemistry meiotic arrest Fertility Receptors Androgen blood-testis barrier integrity Testis Animals Physical and Theoretical Chemistry Spermatogenesis Molecular Biology Blood-Testis Barrier [SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis Spectroscopy |
Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 11; Pages: 5893 International Journal of Molecular Sciences International Journal of Molecular Sciences, 2022, 23 (11), pp.5893. ⟨10.3390/ijms23115893⟩ |
ISSN: | 1422-0067 1661-6596 |
DOI: | 10.3390/ijms23115893 |
Popis: | International audience; In vitro spermatogenesis appears to be a promising approach to restore the fertility of childhood cancer survivors. The rat model has proven to be challenging, since germ cell maturation is arrested in organotypic cultures. Here, we report that, despite a meiotic entry, abnormal synaptonemal complexes were found in spermatocytes, and in vitro matured rat prepubertal testicular tissues displayed an immature phenotype. RNA-sequencing analyses highlighted up to 600 differentially expressed genes between in vitro and in vivo conditions, including genes involved in blood-testis barrier (BTB) formation and steroidogenesis. BTB integrity, the expression of two steroidogenic enzymes, and androgen receptors were indeed altered in vitro. Moreover, most of the top 10 predicted upstream regulators of deregulated genes were involved in inflammatory processes or immune cell recruitment. However, none of the three anti-inflammatory molecules tested in this study promoted meiotic progression. By analysing for the first time in vitro matured rat prepubertal testicular tissues at the molecular level, we uncovered the deregulation of several genes and revealed that defective BTB function, altered steroidogenic pathway, and probably inflammation, could be at the origin of meiotic arrest. |
Databáze: | OpenAIRE |
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