How to handle speciose clades? Mass taxon-sampling as a strategy towards illuminating the natural history of Campanula (Campanuloideae)
| Autor: | Andrew A. Crowl, Thomas Raus, Evgeny V. Mavrodiev, Georgia Kamari, Nico Cellinese, Guilhem Mansion, Nursel Ikinci, Marine Oganesian, Rosemarie Haberle, Galip Akaydin, Thomas Borsch, Katharina Fraunhofer, Dimitrios Phitos, Gerald Parolly |
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| Přispěvatelé: | BAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü, İkinci, Nursel |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Paraphyly Plant Phylogenetics Plant Evolution lcsh:Medicine Plant Science 01 natural sciences Polymerase Chain Reaction Molecular Systematics Clade lcsh:Science Phylogeny 0303 health sciences Likelihood Functions Multidisciplinary biology Campanulaceae Classification Phylogenetics Macroevolution Taxonomy (biology) Research Article Campanula DNA Plant Zoology Forms of Evolution 010603 evolutionary biology Models Biological Evolution Molecular 03 medical and health sciences Campanula (Campanuloideae) Evolutionary Systematics Molecular Biology Biology 030304 developmental biology Taxonomy Evolutionary Biology Models Genetic lcsh:R Botany Species diversity Bayes Theorem Plant Taxonomy 15. Life on land biology.organism_classification Introns Organismal Evolution Phylogeography Evolutionary biology lcsh:Q |
| Zdroj: | PLoS ONE, Vol 7, Iss 11, p e50076 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | WOS:000312601700032 PubMed: 23209646 Background: Speciose clades usually harbor species with a broad spectrum of adaptive strategies and complex distribution patterns, and thus constitute ideal systems to disentangle biotic and abiotic causes underlying species diversification. The delimitation of such study systems to test evolutionary hypotheses is difficult because they often rely on artificial genus concepts as starting points. One of the most prominent examples is the bellflower genus Campanula with some 420 species, but up to 600 species when including all lineages to which Campanula is paraphyletic. We generated a large alignment of petD group II intron sequences to include more than 70% of described species as a reference. By comparison with partial data sets we could then assess the impact of selective taxon sampling strategies on phylogenetic reconstruction and subsequent evolutionary conclusions. Methodology/Principal Findings: Phylogenetic analyses based on maximum parsimony (PAUP, PRAP), Bayesian inference (MrBayes), and maximum likelihood (RAxML) were first carried out on the large reference data set (D680). Parameters including tree topology, branch support, and age estimates, were then compared to those obtained from smaller data sets resulting from "classification-guided'' (D088) and "phylogeny-guided sampling'' (D101). Analyses of D088 failed to fully recover the phylogenetic diversity in Campanula, whereas D101 inferred significantly different branch support and age estimates. Conclusions/Significance: A short genomic region with high phylogenetic utility allowed us to easily generate a comprehensive phylogenetic framework for the speciose Campanula clade. Our approach recovered 17 well-supported and circumscribed sub-lineages. Knowing these will be instrumental for developing more specific evolutionary hypotheses and guide future research, we highlight the predictive value of a mass taxon-sampling strategy as a first essential step towards illuminating the detailed evolutionary history of diverse clades. |
| Databáze: | OpenAIRE |
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