Landscape of heart proteome changes in a diet-induced obesity model

Autor: Paula Grippa Sant’Ana, Dijon Henrique Salomé de Campos, Lucilene Delazari dos Santos, Carlos Roberto Padovani, Paula Paccielli Freire, Robert J. Beynon, Danielle Fernandes Vileigas, Victoria M. Harman, Vitor Loureiro da Silva, Antonio Carlos Cicogna, Cecília Lume de Carvalho Marciano, Sérgio Luiz Borges de Souza, Gustavo Augusto Ferreira Mota, Katashi Okoshi
Přispěvatelé: Universidade Estadual Paulista (Unesp), Liverpool
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019)
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
SCIENTIFIC REPORTS
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-019-54522-2
Popis: Made available in DSpace on 2020-12-12T01:48:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Obesity is a pandemic associated with a high incidence of cardiovascular disease; however, the mechanisms are not fully elucidated. Proteomics may provide a more in-depth understanding of the pathophysiological mechanisms and contribute to the identification of potential therapeutic targets. Thus, our study evaluated myocardial protein expression in healthy and obese rats, employing two proteomic approaches. Male Wistar rats were established in two groups (n = 13/group): control diet and Western diet fed for 41 weeks. Obesity was determined by the adipose index, and cardiac function was evaluated in vivo by echocardiogram and in vitro by isolated papillary muscle analysis. Proteomics was based on two-dimensional gel electrophoresis (2-DE) along with mass spectrometry identification, and shotgun proteomics with label-free quantification. The Western diet was efficient in triggering obesity and impaired contractile function in vitro; however, no cardiac dysfunction was observed in vivo. The combination of two proteomic approaches was able to increase the cardiac proteomic map and to identify 82 differentially expressed proteins involved in different biological processes, mainly metabolism. Furthermore, the data also indicated a cardiac alteration in fatty acids transport, antioxidant defence, cytoskeleton, and proteasome complex, which have not previously been associated with obesity. Thus, we define a robust alteration in the myocardial proteome of diet-induced obese rats, even before functional impairment could be detected in vivo by echocardiogram. Department of Internal Medicine Botucatu Medical School São Paulo State University (UNESP) Centre for Proteome Research Institute of Integrative Biology University of Liverpool Liverpool Department of Morphology Institute of Biosciences São Paulo State University (UNESP) Department of Biostatistics Institute of Biosciences São Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP)/Graduate Program in Tropical Diseases (FMB) São Paulo State University (UNESP) Department of Internal Medicine Botucatu Medical School São Paulo State University (UNESP) Department of Morphology Institute of Biosciences São Paulo State University (UNESP) Department of Biostatistics Institute of Biosciences São Paulo State University (UNESP) Center for the Study of Venoms and Venomous Animals (CEVAP)/Graduate Program in Tropical Diseases (FMB) São Paulo State University (UNESP) FAPESP: 2014/22152-0 FAPESP: 2015/16934-8 FAPESP: 2017/09688-6
Databáze: OpenAIRE
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