Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry
| Autor: | Mariana Z. T. de Lima, Wanius Garcia, Leonardo R. Almeida, Amanda Bernardes, João R. C. Muniz, Alain M. Mera |
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| Rok vydání: | 2021 |
| Předmět: |
Sucrose
biology Prebiotic medicine.medical_treatment food and beverages Fructose General Chemistry biology.organism_classification Lactobacillus gasseri Lactobacillus reuteri chemistry.chemical_compound Fructan chemistry Hydrolase medicine BIOQUÍMICA Fermentation Food science General Agricultural and Biological Sciences |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from L. gasseri belonging to the GH32 family. Apo-LgAs32 and LgAs32 complexed with β-d-fructose structures were determined at a resolution of 1.94 and 1.84 A, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant LgAs32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from Lactobacillus reuteri, the production of 1-kestose by LgAs32 is increased; thus, LgAs32 can be considered as an alternative in fructan production and other industrial applications. |
| Databáze: | OpenAIRE |
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