Gene expression analysis by cDNA-AFLP highlights a set of new signaling networks and translational control during seed dormancy breaking in Nicotiana plumbaginifolia
| Autor: | Laurent Ogé, Marc Jullien, Jérôme Bove, Béatrice Godin, Philippe Lucas, Philippe Grappin |
|---|---|
| Přispěvatelé: | Biologie des Semences (LBS), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Department of Biology, Pennsylvania State University (Penn State), Penn State System-Penn State System, Institut National Agronomique Paris Grignon (INAPG) |
| Rok vydání: | 2004 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Germination Plant Science Biology ABSCISIC ACID CDNA-AFLP chemistry.chemical_compound Gene Expression Regulation Plant Complementary DNA Gene expression Tobacco Genetics Transcriptional regulation SEED GERMINATION [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Nicotiana plumbaginifolia Abscisic acid Gene Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Seed dormancy food and beverages Gene Expression Regulation Developmental Reproducibility of Results General Medicine Sequence Analysis DNA NICOTIANA PLUMBAGINIFOLIA Blotting Northern chemistry Protein Biosynthesis Seeds Dormancy SEED DORMANCY Agronomy and Crop Science Nucleic Acid Amplification Techniques Signal Transduction |
| Zdroj: | Plant Molecular Biology Plant Molecular Biology, Springer Verlag (Germany), 2005, 57 (4), pp.593-612. ⟨10.1007/s11103-005-0953-8⟩ |
| ISSN: | 0167-4412 |
| DOI: | 10.1007/s11103-005-0953-8⟩ |
| Popis: | Seed dormancy in Nicotiana plumbaginifolia is characterized by an abscisic acid accumulation linked to a pronounced germination delay. Dormancy can be released by 1 year after-ripening treatment. Using a cDNA-amplified fragment length polymorphism (cDNA-AFLP) approach we compared the gene expression patterns of dormant and after-ripened seedS., air-dry or during one day imbibition and analyzed 15,000 cDNA fragments. Among theM., 1020 were found to be differentially regulated by dormancy. Of 412 sequenced cDNA fragmentS., 83 were assigned to a known function by search similarities to public databases. The functional categories of the identified dormancy maintenance and breaking responsive geneS., give evidence that after-ripening turns in the air-dry seed to a new developmental program that modulateS., at the RNA level, components of translational control, signaling networkS., transcriptional control and regulated proteolysis. |
| Databáze: | OpenAIRE |
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