Action of neuwiedase, a metalloproteinase isolated from Bothrops neuwiedi venom, on skeletal muscle: an ultrastructural and immunocytochemistry study
| Autor: | A.O. Gomes, C. Baldo, Veridiana M. Rodrigues, Amélia Hamaguchi, Eloisa Amália Vieira Ferro, Daiana Silva Lopes, Luiz Fernando Moreira Izidoro, M. J. Ferreira, Maria Inês Homsi-Brandeburgo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
lcsh:RC955-962 Bothrops neuwiedi Biology Toxicology Gastrocnemius muscle lcsh:RA1190-1270 Myosin lcsh:Zoology medicine Myocyte snake venom metalloproteinases lcsh:QL1-991 skeletal muscle lcsh:Toxicology. Poisons Metalloproteinase Skeletal muscle neuwiedase musculoskeletal system Cell biology Infectious Diseases medicine.anatomical_structure Biochemistry Snake venom Animal Science and Zoology Parasitology Basal lamina Myofibril |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 462-469 (2010) |
| ISSN: | 1678-9199 |
| Popis: | The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through myofibrils extracted from gastrocnemius muscle indicated that neuwiedase provoked degradation of myofibrils, especially myosin. These results suggest that skeletal muscle damage, induced by neuwiedase, is probably due to its proteolytic action on myofibrils, which are responsible for the maintenance of the cellular architecture. |
| Databáze: | OpenAIRE |
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