Eupatilin suppresses the allergic inflammatory response in vitro and in vivo
| Autor: | Yun-Mi Kang, Minho Lee, Hyo-Jin An, Kyung-Sook Chung, Jong-Hyun Lee, Eun-Hye Song |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine MAPK/ERK pathway medicine.medical_specialty p38 mitogen-activated protein kinases Eupatilin medicine.medical_treatment Pharmaceutical Science Pharmacology Histamine Release Cell Line Allergic inflammation 03 medical and health sciences chemistry.chemical_compound NF-KappaB Inhibitor alpha Internal medicine Anti-Allergic Agents Drug Discovery medicine Animals Humans p-Methoxy-N-methylphenethylamine Mast Cells Phosphorylation Anaphylaxis Flavonoids Mice Inbred ICR business.industry Anti-Inflammatory Agents Non-Steroidal NF-kappa B NF-κB IκBα 030104 developmental biology Endocrinology Cytokine Complementary and alternative medicine chemistry Cytokines Tetradecanoylphorbol Acetate Molecular Medicine Tumor necrosis factor alpha business medicine.drug |
| Zdroj: | Phytomedicine. 42:1-8 |
| ISSN: | 0944-7113 |
| Popis: | Introduction Eupatilin, a pharmacologically active ingredient found in Artemisia asiatica, has been reported to have anti-oxidative, anti-inflammatory, and anti-apoptotic activities. However, molecular mechanisms underlying its anti-allergic properties are not yet clear. In this study, we investigated the effects of eupatilin on allergic inflammation in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells and a compound 48/80-induced anaphylactic shock model. Methods Cytokine assays, histamine assays, quantitative real-time polymerase chain reaction analysis, western blot analysis and compound 48/80-induced anaphylactic shock model were used in this study. Results Eupatilin significantly suppresses the expression and production of pro-inflammatory cytokines, such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 in vitro and in vivo. In addition, eupatilin inhibits nuclear factor kappa B (NF-κB) activation by regulating the phosphorylation and degradation of IκBα via the Akt/IKK(α/β) pathway. Eupatilin treatment also attenuates the phosphorylation of p38, ERK, and JNK MAPKs. Furthermore, eupatilin blocked anaphylactic shock and decreased the release of histamine. Conclusions Anti-allergic inflammation may involve the expression and production of regulating pro-inflammatory cytokines via Akt/IKK(α/β) and MAPK activation of NF-κB. On the basis of these data, eupatilin is a potential candidate for the treatment of allergic diseases. |
| Databáze: | OpenAIRE |
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