Antiproliferative effect of 1,4-phenylenebis(methylene)selenocyanate (p-XSC) on colonic epithelium of patients with adenomatous polyps in vitro
| Autor: | Gerda Dusel, Pramod Upadhyaya, Karam El-Bayoumy, S. Krüger, Wolfgang Scheppach, H P Bartram |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
medicine.medical_specialty Epidemiology Colon Biopsy Crypt Cell Culture Techniques Gastroenterology chemistry.chemical_compound Adenomatous Polyps Risk Factors Internal medicine Organoselenium Compounds medicine Anticarcinogenic Agents Humans Intestinal Mucosa Incubation Dose-Response Relationship Drug Cell growth Public Health Environmental and Occupational Health Cancer medicine.disease Molecular biology Immunohistochemistry In vitro Epithelium medicine.anatomical_structure Oncology chemistry Colonic Neoplasms Bromodeoxyuridine Biomarkers Cell Division |
| Zdroj: | European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP). 12(6) |
| ISSN: | 0959-8278 |
| Popis: | We have consistently shown that the organoselenium compound 1,4-phenylenebis(methylene)selenocyanate (p-XSC) is a superior cancer chemopreventive agent and less toxic than selenite or certain naturally-occurring selenoamino acids. To elucidate the effects of p-XSC on human colonic mucosa, biopsies from endoscopically normal sigmoid colon of 30 patients with adenomatous polyps were incubated with p-XSC at concentrations of 1, 2 and 5 micromol/l dissolved in dimethylsulphoxide (DMSO). Biopsies incubated with DMSO or pure culture medium served as a control. Proliferating cells were labelled by bromodeoxyuridine immunohistochemistry and the labelling index (LI) was computed. Upper crypt labelling index (LI of crypt compartments 4+5) and Phih value, which are both discriminators of the expansion of the proliferative zone, were significantly lower after incubation with 1 and 5 micromol/l p-XSC, respectively (LI 4+5: 0.8 and 1.0; Phih value: 2.1 and 2.4), as compared with DMSO (LI 4+5: 3.6 and 4.5; Phih value: 7.0 and 8.3) or culture medium (LI 4+5: 3.3 and 4.5; Phih value: 7.2 and 8.1) (P |
| Databáze: | OpenAIRE |
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