The role of oxidative stress in alterations of hematological parameters and inflammatory markers induced by early hypercholesterolemia
| Autor: | María Peral de Bruno, Liliana Sierra, María Sofía Karbiner, Carlos Minahk, Susana Jerez, María Cristina Fonio |
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| Rok vydání: | 2013 |
| Předmět: |
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medicine.medical_specialty CIENCIAS MÉDICAS Y DE LA SALUD Membrane Fluidity Hypercholesterolemia Biology medicine.disease_cause Antioxidants General Biochemistry Genetics and Molecular Biology Cholesterol Dietary Internal medicine medicine Animals Stress oxidativo General Pharmacology Toxicology and Pharmaceutics Hematologia Hipercolesterolemia General Medicine Bioquímica y Biología Molecular Blood Cell Count Osmotic Fragility Oxidative Stress Medicina Básica Endocrinology Conejos Rabbits Inflammation Mediators Biomarkers Oxidative stress |
| Zdroj: | Life Sciences. 93:503-508 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2013.08.003 |
| Popis: | Aims: The investigation of the effects of a high cholesterol diet (HD) for a short-time period on hematological parameters and the potential role of oxidative stress and inflammation markers. Main methods: Rabbits were fed either a control diet or a diet containing 1% cholesterol (HD) for 5–6 weeks. The plasma lipid levels, C reactive protein (CRP), total red blood cells (RBC), total white blood cells (WBC), platelet count, packed cell volume (PCV) and leukocyte formula were determined. Oxidative stress was evaluated by the thiobarbituric acid reactive substances (TBARS), total glutathione and GSH serum level measurements. The osmotic fragility and the membrane fluidity of erythrocytes were determined. The levels of total cholesterol and TBARS were also measured in the erythrocyte membrane suspension. Key findings: A decrease in the RBC and PCV was observed in rabbits fed on HD. The membrane rigidity and osmotic fragility were increased, and the morphological changes caused by the HD and TBARS levels in the erythrocyte membrane may account for this phenomenon. The inflammatory markers as the CRP levels, the platelet count, the WBC and the neutrophils were increased. The TBARS and GSH levels in the serum were increased and decreased, respectively. Significance: This study shows that feeding rabbits an HD for a short time induces hematological alterations, disturbances in the oxidant–antioxidant balance and an increase of inflammatory markers. These findings support the importance of the early correction or prevention of high cholesterol levels to disrupt the process leading to the development of cardiovascular diseases. Fil: Karbiner, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Sierra, Liliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Minahk, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Fonio, Maria Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Bioquímica Aplicada; Argentina Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Jerez, Susana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina |
| Databáze: | OpenAIRE |
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