Spermatozoa recovery and post-thawing quality of brown bear ejaculates is affected for centrifugation regimes
| Autor: | Mercedes Alvarez, M. Nicolas, E. Anel, S. Borragan, Paulino de Paz, F. Martínez, Luis Anel, Felipe Martínez-Pastor |
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| Přispěvatelé: | Biologia Celular, Facultad de Ciencias Biologicas y Ambientales |
| Rok vydání: | 2011 |
| Předmět: |
endocrine system
food.ingredient Centrifugation Semen Management Monitoring Policy and Law Biology Electroejaculation Cryopreservation law.invention Andrology food law Yolk Botany Ursus Ecology Evolution Behavior and Systematics Nature and Landscape Conservation urogenital system Extender biology.organism_classification Spermatozoa Sperm Brown bear Veterinaria |
| Zdroj: | European Journal of Wildlife Research. 58:77-84 |
| ISSN: | 1439-0574 1612-4642 |
| DOI: | 10.1007/s10344-011-0544-8 |
| Popis: | P. 77–84 Sperm cryopreservation protocols for brown bear (Ursus arctos) require the centrifugation of semen samples to increase sperm concentration and to clean urine in contaminated samples. We evaluated the effect of centrifugation regimes (time and relative centrifugal force—RCF) on the quantity of sperm recovered and the quality of post-thawed sperm. Thirteen brown bears were electroejaculated. The ejaculates were diluted 1:1 in Tris–citric acid–glucose (TCG) extender and centrifuged with different RCF/time combinations: 600×g, 1,200×g and 2,400×g, for 3, 6 or 12 min. After centrifugation, spermatozoa were diluted in TES–Tris–fructose extender with egg yolk and glycerol (final glycerol concentration of 8%) and frozen in 0.25-mL straws. In the post-thawed semen, motility was assessed by CASA, and acrosomal status (PNA-FITC), viability (SYBR-14 with propidium iodide) and chromatin status (SCSA) were determined by flow cytometry. The longest centrifugation time (12 min) significantly decreased some motility parameters. Sperm recovery significantly decreased in brown bear at 600×g. Our results suggest that brown bear spermatozoa are more sensitive to long centrifugation times than to high RCF. Centrifugation regimes showed no effects on the post-thawing chromatin status. We recommend preparing the brown bear semen for freezing by centrifugation 1,200×g or 2,400×g for 6 min, after electroejaculation and dilution 1:1 in TCG extender, since these procedures increase the spermatozoa recovery without harmful effects on the post-thawed quality of brown bear spermatozoa. SI |
| Databáze: | OpenAIRE |
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