Prostaglandin synthesis can be inhibited locally by infusion of NSAIDS through microdialysis catheters in human skeletal muscle
| Autor: | Michael Kjaer, Henning Langberg, Ulla Ramer Mikkelsen, Ida C. Helmark |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Microdialysis Time Factors Physiology Indomethacin Down-Regulation Prostaglandin Inflammation Dinoprostone Quadriceps Muscle chemistry.chemical_compound Catheters Indwelling Physiology (medical) Internal medicine medicine Humans Cyclooxygenase Inhibitors Infusions Parenteral Prostaglandin E2 Exercise Anti-Inflammatory Agents Non-Steroidal Skeletal muscle Recovery of Function Blood flow Endocrinology medicine.anatomical_structure Eicosanoid chemistry medicine.symptom Muscle Contraction medicine.drug Muscle contraction |
| Zdroj: | Journal of Applied Physiology. 104:534-537 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.01016.2007 |
| Popis: | Prostaglandins are known to be involved in the regulation of local blood flow within human skeletal muscles during exercise, and the concentration of prostaglandins increases locally and systemically in response to exercise. The systemic release of prostaglandins can be inhibited by oral intake of nonsteroidal anti-inflammatory drugs (NSAIDs). However, to study the local role of prostaglandins, the formation of prostaglandins within the tissue must be controlled. Microdialysis enables determination of local concentrations of water-soluble substances within the tissue. In the present study, the microdialysis method was used to infuse NSAIDs locally into human skeletal muscles producing a local block of prostaglandin formation. In addition, the graded blockade at various distances from the infusion site within the muscle during rest, exercise and recovery was determined. Microdialysis was performed in thigh muscles (vastus lateralis muscle) in six healthy men. One of the microdialysis catheters was used to block prostaglandin synthesis by infusion of the NSAID indomethacin. Additional catheters were placed 1 and 4 cm away from the infusion and in the contralateral leg (working control). Following 2 h of rest, the subjects performed 200 maximal eccentric contractions with each leg followed by 3 h of rest. The study revealed that infusion of NSAID reduced local prostaglandin E2 concentration by ∼30–50% (4 cm away from the infusion) and 85% (1 cm away from the infusion) compared with the contralateral (unblocked) thigh muscle. In conclusion, the present study shows that infusion of NSAIDs into human muscle via microdialysis catheters results in a graded blockade of prostaglandin synthesis. |
| Databáze: | OpenAIRE |
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