Synthetic biological circuit tested in spaceflight
| Autor: | Chris Welch, Yadvender Dhillon, Claire Villette, James Bevington, Ming C. Hammond, Robert N. Bowman, Jean-Jacques Favier, Philippe Giegé, Laurence Drouard, Dimitri Heintz, Barnaby Osborne, Christopher P. McKay, Mera F. Horne, Mike Manefield, Rebekah Z. Kitto, Nicolas Corre, Mathilde Arrivé |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
010504 meteorology & atmospheric sciences
Health Toxicology and Mutagenesis [SDV]Life Sciences [q-bio] Arabidopsis Computational biology Biology Spaceflight 01 natural sciences law.invention Synthetic biology RNA interference law 0103 physical sciences Arabidopsis thaliana 010303 astronomy & astrophysics Transcription factor ComputingMilieux_MISCELLANEOUS 0105 earth and related environmental sciences Radiation Estradiol Integrases Ecology Arabidopsis Proteins Polymerase chain reaction analysis Astronomy and Astrophysics Space Flight Plants Genetically Modified biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Plant Leaves Receptors Estrogen RNA Plant RNA Interference Synthetic Biology Transcription Factors |
| Zdroj: | Life Sciences in Space Research Life Sciences in Space Research, Elsevier 2020, ⟨10.1016/j.lssr.2020.09.002⟩ |
| ISSN: | 2214-5524 |
| DOI: | 10.1016/j.lssr.2020.09.002⟩ |
| Popis: | Synthetic biology has potential spaceflight applications yet few if any studies have attempted to translate Earth-based synthetic biology tools into spaceflight. An exogenously inducible biological circuit for protein production in Arabidopsis thaliana, pX7-AtPDSi (Guo et al. 2003), was flown to ISS and functionally investigated. Seedlings were grown in a custom built 1.25 U plant greenhouse. Images recorded during the experiment show that leaves of pX7-AtPDSi seedlings photobleached as designed while wild type Col-0 leaves did not, which reveals that the synthetic circuit led to protein production during spaceflight. Polymerase chain reaction analysis post-flight also confirms that the Cre/LoxP (recombination system) portions of the circuit were functional in spaceflight. The subcomponents of the biological circuit, estrogen-responsive transcription factor XVE, Cre/LoxP DNA recombination system, and RNAi post-transcriptional gene silencing system now have flight heritage and can be incorporated in future designs for space applications. To facilitate future plant studies in space, the full payload design and manufacturing files are made available. |
| Databáze: | OpenAIRE |
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