Seroprevalence of Bluetongue Virus in small ruminants in Balochistan province, Pakistan

Autor:	Nadia Mukhtar, Momena Habib, Muhammad Hamid Chaudhary, Aamir Ghafoor, Tahir Yaqub, Masood Rabbani, Jawad Nazir, Tariq Abbas, Tehmina Sohail, Muhammad Shafee, Muhammad Yasir Zahoor, Muhammad Zubair Shabbir, N. Ullah, A. Ul Rahman, Amir Iftikhar Malik
Rok vydání:	2018
Předmět:	0301 basic medicine Serotype Veterinary medicine 040301 veterinary sciences Sheep Diseases Enzyme-Linked Immunosorbent Assay Biology Antibodies Viral Real-Time Polymerase Chain Reaction Serogroup Bluetongue Virus 0403 veterinary science 03 medical and health sciences Seroepidemiologic Studies Odds Ratio Prevalence Animals Seroprevalence Pakistan Seroconversion Breed type Disease burden Goat Diseases Sheep General Veterinary General Immunology and Microbiology Goats Viral Core Proteins 04 agricultural and veterinary sciences General Medicine Odds ratio Cross-Sectional Studies 030104 developmental biology Herd Bluetongue virus
Zdroj:	Transboundary and Emerging Diseases. 65:1272-1281
ISSN:	1865-1674
DOI:	10.1111/tbed.12871
Popis:	Bluetongue (BT), caused by bluetongue virus (BTV), is a vector-borne disease of small ruminants that has the potential to spread across international borders. Despite large populations of susceptible animals and borders with BTV endemic countries, little is known of the disease burden and prevalent serotypes in the province of Balochistan in Pakistan. We conducted a cross-sectional study to determine seroconversion and prevalent serotypes in selected districts of the province using a competitive enzyme-linked immunosorbent assay (cELISA) and real-time polymerase chain reaction (RT-PCR). Sera (n = 876) were collected from clinically healthy sheep and goats originating from the districts of Quetta (n = 300), Mastung (n = 201), Killa Saifullah (n = 75) and Kech (n = 300). None of the study herds (n = 97) were seronegative for BTV, and at the individual level, the overall prevalence of BTV seroconversion was 47.26% (n = 414/876, 95% CI = 43.92%-50.63%). A higher percentage of goats (50.87%, 95% CI = 45.99%-55.73%) were seropositive for anti-VP7 immunoglobulins (IgG) than sheep (44.21%, 95% CI = 39.81%-48.70%). Odds ratios of seroconversion for goats were associated with breed type (χ2 = 16.84, p = .01), parity (χ2 = 23.66, p = .00) and presence of vector (χ2 = 2.63, p = .10), whereas for sheep, it was associated with breed type (χ2 = 13.80, p = .01) and parity (χ2 = 53.40, p = .00). Serotype 8 was the most prevalent (26.82%, 95% CI = 14.75%-43.21%) followed by an equal prevalence of serotypes 2 and 9 (7.31%, 95% CI = 1.91%-21.01%). To the best of our knowledge, this is the first study conducted in Balochistan province and the results indicate that there is a necessity to initiate intervention strategies to control BT disease burden not only in this region of Pakistan but also in adjacent areas of the neighbouring countries, Iran and Afghanistan.
Databáze:	OpenAIRE
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