Genetic diversity and population structure of mangabeira (Hancornia speciosa) estimated using ISSR markers

Autor: Paulo Sarmanho da Costa Lima, Maria Clideana Cabral Maia, Jailson de Araújo Santos, Gizele de Andrade Luz, Kelly Pires de Oliveira, Pâmela Ponce Martins, Sérgio Emílio dos Santos Valente
Přispěvatelé: GIZELE DE ANDRADE LUZ, UFPI, JAILSON DE ARAÚJO SANTOS, UFPI, KELLY PIRES DE OLIVEIRA, UESPI, PÂMELA PONCE MARTINS, UFPI, SÉRGIO EMÍLIO DOS SANTOS VALENTE, UESPI, MARIA CLIDEANA CABRAL MAIA, CNPAT, PAULO SARMANHO DA COSTA LIMA, CPAMN.
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Acta Scientiarum. Biological Sciences; Vol 42 (2020): Publicação contínua; e53606
Acta Scientiarum. Biological Sciences; v. 42 (2020): Publicação contínua; e53606
Acta Scientiarum Biological Sciences
Universidade Estadual de Maringá (UEM)
instacron:UEM
Acta Scientiarum : Biological Sciences, Vol 42, Pp e53606-e53606 (2020)
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice)
Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
ISSN: 1807-863X
1679-9283
Popis: The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species. The mangabeira is a native fruit tree from Brazil with fruits that present significant potential for exploitation. This species is experiencing genetic erosion, which increases the importance of elucidating the genetic diversity that exists in mangabeira populations to support conservation programs. Thus, this study aimed to evaluate the diversity and genetic structure of mangabeira populations from the Germplasm Bank of Embrapa Meio-Norte using inter simple sequence repeat (ISSR) markers. A total of 29 accessions from Brazil were characterized, including one from Sergipe, one from Bahia, three from the Distrito Federal, 11 from Piauí and 13 from Paraíba. The 11 ISSR primers provided 166 loci, among which 120 were polymorphic. The analysis of molecular variance (AMOVA) indicated that 69.66% of the observed genetic variability occurred within populations and that the populations showed high genetic differentiation. The results obtained from the STRUCTURE analysis indicated the existence of two genetic groups. The Nei and Shannon indices of genetic diversity varied from 0.15 to 0.24 and from 0.22 to 0.34, respectively. The coefficient of similarity ranged from 0.57 to 0.94, with a mean of 0.76. The mean was used as the cut-off point in the dendrogram, and seven groups were identified. In conclusion, this study demonstrates the presence of low or moderate genetic diversity within the studied mangabeira populations and high genetic differentiation between the populations. The results indicate a need to increase the number of mangabeira population samples from different collection sites as a strategy to achieve more significant results for the conservation and genetic improvement of this species.
Databáze: OpenAIRE
Externí odkaz: