Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method
| Autor: | Hiroko Kobayashi, Tao Zhuang, Hisashi Aso, Yuko Sato, Yoshio Kiku, Teruaki Taguchi, Megumi Ito, Kouichi Watanabe, Yuya Nagasawa, Megumi Urakawa, Haruki Kitazawa, Naokazu Takada, Michael T. Rose, Tomonori Nochi, Shiro Katto, Hidetoshi Sato, Tomohito Hayashi, Tsuyoshi Umino, Kozue Yoshimura, Koutaro Tanaka |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
dendritic cell phenotype chemical and pharmacologic phenomena Cell Separation Adaptive Immunity Major histocompatibility complex Peripheral blood mononuclear cell Physiology/Anatomy Immune tolerance Inducible T-Cell Co-Stimulator Protein 03 medical and health sciences 0302 clinical medicine Pregnancy cytokine Immune Tolerance Animals Humans Antigen-presenting cell MHC class II Antigen Presentation CD40 biology Parturition hemic and immune systems General Medicine Dendritic cell Dendritic Cells Acquired immune system Immunity Innate Cell biology 030104 developmental biology cattle biology.protein Leukocytes Mononuclear Cytokines Original Article positive‐selection Female ORIGINAL ARTICLES General Agricultural and Biological Sciences 030215 immunology |
| Zdroj: | Animal Science Journal = Nihon Chikusan Gakkaiho SC10201810160014 NARO成果DBa |
| ISSN: | 1740-0929 1344-3941 |
| Popis: | Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a+/CD11c+/MHC (major histocompatibility complex) class II + cells. Although DCs are identified at 0.1%–0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic‐activated cell sorting, and purified the CD172a+/CD11c+ DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co‐stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co‐stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co‐stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co‐stimulatory molecules may already have the ability of modulating the T‐cell linage. |
| Databáze: | OpenAIRE |
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