Narrow bore post column derivatisation assays via reaction flow chromatography
| Autor: | R. Andrew Shalliker, Andrew Jones, Agustín Acquaviva, Gary R. Dennis, Arianne Soliven, Cecilia B. Castells |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Flow (psychology) NARROW BORE Mixing (process engineering) 02 engineering and technology 01 natural sciences High-performance liquid chromatography Column (database) Noise (electronics) Analytical Chemistry REACTION FLOW CHROMATOGRAPHY POST COLUMN DERIVATISATION PHENOLICS Spectroscopy Coupling Chromatography SELECTIVE DETECTION 010401 analytical chemistry Ciencias Químicas 021001 nanoscience & nanotechnology 0104 chemical sciences SCALED DOWN Química Analítica 0210 nano-technology Sensitivity (electronics) Frit CIENCIAS NATURALES Y EXACTAS |
| Popis: | This study highlights the capability to conduct a post column derivatisation (PCD) process with a narrow bore, low volume column. A reaction flow (RF) HPLC column (30 mm × 2.1 mm internal diameter (i.d.), 3 μm particle diameter) enabled the selective detection of phenols for both a simple standard mixture and a complex sample represented by green tea. The resolving power and sensitivity obtained were similar to an analytical scale (4.6 mm i.d.) RF HPLC column of the same format. However, the baseline noise experienced was higher when the assay was scaled down from the RF 4.6 mm i.d. to the RF 2.1 mm i.d. narrow bore column. The PCD mixing and reaction speed inside the RF column outlet (frit and head-fitting design) enabled the coupling of PCD protocols with “UHPLC-type” low volume columns and systems without significant loss in separation performance. In comparison to a conventional analytical scale PCD approach the scaled down narrow bore RF approach demonstrated 40 times greater sensitivity in terms of S/N. Scaled down PCD is vital for decreased consumption and cost without significant loss in separation performance. Fil: Acquaviva, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina Fil: Jones, Andrew. Western Sydney University; Australia Fil: Dennis, Gary R.. Western Sydney University; Australia Fil: Castells, Cecilia Beatriz Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Facultad de Ciencias Exactas, Universidad Nacional de la Plata; Argentina Fil: Shalliker, R. Andrew. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Western Sydney University; Australia Fil: Soliven, Arianne. Universidad de la República; Uruguay |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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