Molecular epidemiology of endemic ciprofloxacin-resistant Neisseria gonorrhoeae in Liverpool

Autor: A J Komolafe, J E Corkill, C A Hart, Timothy Neal, A B Alawattegama, A Mortimore
Rok vydání: 2003
Předmět:
Serotype
DNA Topoisomerase IV
DNA
Bacterial
Male
Endemic Diseases
Dermatology
Drug resistance
Microbial Sensitivity Tests
medicine.disease_cause
Polymerase Chain Reaction
law.invention
Microbiology
Gonorrhea
Anti-Infective Agents
law
Ciprofloxacin
Genotype
Drug Resistance
Bacterial
medicine
Humans
Point Mutation
Pharmacology (medical)
Serotyping
Medical History Taking
Polymerase chain reaction
Molecular Epidemiology
Molecular epidemiology
biology
Ceftriaxone
Public Health
Environmental and Occupational Health
biology.organism_classification
DNA Fingerprinting
Neisseria gonorrhoeae
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Infectious Diseases
DNA profiling
Amino Acid Substitution
England
DNA Gyrase
Neisseriaceae
Female
Polymorphism
Restriction Fragment Length
Bacterial Outer Membrane Proteins
Zdroj: International journal of STDAIDS. 14(6)
ISSN: 0956-4624
Popis: Ciprofloxacin-resistant Neisseria gonorrhoeae had been rarely detected on Merseyside and when found was associated with β-lactamase producing strains, imported from abroad. However, in August 2000, two cases of infection with ciprofloxacin-resistant β-lactamase-negative strains occurred in sexually unrelated patients with no history of foreign travel. Over the next 18 months a total of 120 patients presented with ciprofloxacin-resistant gonococci, from which 99 patient strains were available for study. Gonococcal DNA was subjected to molecular fingerprinting by polymerase chain reaction amplification followed by Taq1 digestion of their opa genes. Twelve differing opa-types were found, but 79 patients were infected with a single genotype, opa-type 1. The sexual histories of the majority of this group indicated acquisition in Merseyside. This endemic strain was further characterized by having the same amino acid substitutions on gyrA and parC genes. An endemic clone of ciprofloxacin-resistant N. gonorrhoeae has been established on Merseyside necessitating the introduction of ceftriaxone as first-line treatment. Despite the presence of 11 other clones in the city, opa type-1 strains have not yet been displaced, raising the possibility that this strain is endowed with added virulence/endemicity traits or that a number of source patients have not yet been found.
Databáze: OpenAIRE
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