Macromolecular structure and film properties of enzymatically-engineered high molar mass dextrans
Autor: | Florent Grimaud, Gabrielle Potocki-Véronèse, Pauline Faucard, Claire Moulis, Jean-Eudes Maigret, Magali Remaud-Simeon, Jean-Luc Putaux, Denis Lourdin, Agnès Rolland-Sabaté |
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Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Toulouse White Biotechnology (TWB), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR 14-CE27-0011-02, Centre de Recherches sur les Macromolécules Végétales (CERMAV), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS) |
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Sucrose Polymers and Plastics Macromolecular Substances hygromechanical properties Dispersity molecular structure 010402 general chemistry Branching (polymer chemistry) 01 natural sciences sucrose-active enzymes structure moléculaire 03 medical and health sciences chemistry.chemical_compound polymère Elastic Modulus Polymer chemistry Materials Chemistry Transition Temperature Solubility thermal characteristic Molar mass Calorimetry Differential Scanning dextrans Organic Chemistry thermal properties Water Humidity asymmetrical flow field-flow fractionation Dynamic mechanical analysis 0104 chemical sciences Molecular Weight 030104 developmental biology Dextran [CHIM.POLY]Chemical Sciences/Polymers chemistry Chemical engineering Glucosyltransferases Hydrodynamics Radius of gyration propriété thermique Glass Glass transition Sucrase |
Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2018, 181, pp.337-344. ⟨10.1016/j.carbpol.2017.10.065⟩ Carbohydrate Polymers, 2018, 181, pp.337-344. ⟨10.1016/j.carbpol.2017.10.065⟩ |
ISSN: | 0144-8617 |
DOI: | 10.1016/j.carbpol.2017.10.065⟩ |
Popis: | New α(1 → 2) or α(1 → 3) branched dextrans with high molar masses and controlled architecture were synthesized using a dextransucrase and branching sucrases. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties were determined. These new dextrans present structures with low densities from 9,500 to 14,000 g m−3 in H2O/DMSO medium, their molar mass, size and dispersity increase with increasing branching degree (weight-average molar mass up to 109 g mol−1 and radius of gyration around 500 nm). Dextrans exhibit a glass transition between 40.5 and 63.2 °C for water content varying from 12.2 to 14.1%. The effect of branching is mainly observed on the ability of dextran to crystallize. They have a good film-forming ability with a storage modulus which varies from 2 to 4 GPa within a relative humidity range of 10–50%. |
Databáze: | OpenAIRE |
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