Macromolecular structure and film properties of enzymatically-engineered high molar mass dextrans

Autor: Florent Grimaud, Gabrielle Potocki-Véronèse, Pauline Faucard, Claire Moulis, Jean-Eudes Maigret, Magali Remaud-Simeon, Jean-Luc Putaux, Denis Lourdin, Agnès Rolland-Sabaté
Přispěvatelé: Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Toulouse White Biotechnology (TWB), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre de Recherches sur les Macromolécules Végétales (CERMAV ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), ANR 14-CE27-0011-02, Centre de Recherches sur les Macromolécules Végétales (CERMAV), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Sucrose
Polymers and Plastics
Macromolecular Substances
hygromechanical properties
Dispersity
molecular structure
010402 general chemistry
Branching (polymer chemistry)
01 natural sciences
sucrose-active enzymes
structure moléculaire
03 medical and health sciences
chemistry.chemical_compound
polymère
Elastic Modulus
Polymer chemistry
Materials Chemistry
Transition Temperature
Solubility
thermal characteristic
Molar mass
Calorimetry
Differential Scanning

dextrans
Organic Chemistry
thermal properties
Water
Humidity
asymmetrical flow field-flow fractionation
Dynamic mechanical analysis
0104 chemical sciences
Molecular Weight
030104 developmental biology
Dextran
[CHIM.POLY]Chemical Sciences/Polymers
chemistry
Chemical engineering
Glucosyltransferases
Hydrodynamics
Radius of gyration
propriété thermique
Glass
Glass transition
Sucrase
Zdroj: Carbohydrate Polymers
Carbohydrate Polymers, Elsevier, 2018, 181, pp.337-344. ⟨10.1016/j.carbpol.2017.10.065⟩
Carbohydrate Polymers, 2018, 181, pp.337-344. ⟨10.1016/j.carbpol.2017.10.065⟩
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2017.10.065⟩
Popis: New α(1 → 2) or α(1 → 3) branched dextrans with high molar masses and controlled architecture were synthesized using a dextransucrase and branching sucrases. Their molecular structure, solubility, conformation, film-forming ability, as well as their thermal and mechanical properties were determined. These new dextrans present structures with low densities from 9,500 to 14,000 g m−3 in H2O/DMSO medium, their molar mass, size and dispersity increase with increasing branching degree (weight-average molar mass up to 109 g mol−1 and radius of gyration around 500 nm). Dextrans exhibit a glass transition between 40.5 and 63.2 °C for water content varying from 12.2 to 14.1%. The effect of branching is mainly observed on the ability of dextran to crystallize. They have a good film-forming ability with a storage modulus which varies from 2 to 4 GPa within a relative humidity range of 10–50%.
Databáze: OpenAIRE
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