POMC and NPY mRNA expression during development is increased in rat offspring brain from mothers fed with a high fat diet
| Autor: | Luciano Freitas Felício, Ana Carolina Inhasz Kiss, Su-Bin Park, Harry MacKay, Alexander Edwards, Alfonso Abizaid, Marianne O. Klein |
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| Přispěvatelé: | Carleton Univ, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Pro-Opiomelanocortin Offspring Biology Diet High-Fat Metabolic programing 03 medical and health sciences Developmental Neuroscience Pregnancy Internal medicine parasitic diseases Gene expression medicine Animals Offspring development Neuropeptide Y Obesity Rats Wistar Transcription factor Hypothalamic development 2. Zero hunger Fetus Arc (protein) Food intake control Body Weight HIPOTÁLAMO digestive oral and skin physiology Arcuate Nucleus of Hypothalamus Gene Expression Regulation Developmental medicine.disease Rats 030104 developmental biology Endocrinology Hypothalamus Prenatal Exposure Delayed Effects Female Melanocortin Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1873-474X 0736-5748 |
| DOI: | 10.1016/j.ijdevneu.2017.03.004 |
| Popis: | Made available in DSpace on 2018-11-26T17:45:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Natural Sciences and Engineering Research Council of Canada (NSERC) Developmental programing is influenced by perinatal nutrition and it has long-lasting impacts on adult metabolism in the offspring. In particular, maternal high fat diet has been associated with increased risk of obesity and metabolic disorders during adulthood in the descendants. These effects may be due to the effects of the high fat diet on the development of the systems that regulate food intake and energy balance in the offspring hypothalamus. The arcuate nucleus (ARC) may be a particularly sensitive region to it as this nucleus contains the POMC and AgRP/NPY neurons that integrate the melanocortin system. Thus, the aim of this study was to investigate the effects of maternal high fat diet during pregnancy on the transcription factors that regulate hypothalamic development in the offspring as a potential mechanism that may result in altered neuronal expression of POMC, NPY and/or AgRP. To this end, pregnant females exposed to high fat diet (60% fat diet since day 0 of pregnancy) or standard rat chow were sacrificed on days 12, 14, 16 and 18 of gestation to obtain brains from their developing fetuses and examine the mRNA expression of transcription factors associated with the development of cells in the ARC. Results show that, while no changes in transcription factor expression between groups were observed, POMC and NPY mRNA expression were higher on embryonic day 18 in the high fat group. These results suggest that POMC and NPY expression are altered by in utero exposure to a high fat diet, but these changes in gene expression are not associated with changes in the expression of transcription factors known to determine the fate of ARC cells. (C) 2017 ISDN. Published by Elsevier Ltd. All rights reserved. Carleton Univ, Dept Neurosci, 1125 Colonel Dr,Room 329, Ottawa, ON K1S 5B6, Canada Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo, SP, Brazil Sao Paulo State Univ, Dept Physiol, Botucatu, SP, Brazil Univ Sao Paulo, Sch Vet Med, Dept Pathol, Sao Paulo, SP, Brazil Sao Paulo State Univ, Dept Physiol, Botucatu, SP, Brazil FAPESP: 2012/07378-6 FAPESP: 2014/06980-0 Natural Sciences and Engineering Research Council of Canada (NSERC): 341538 |
| Databáze: | OpenAIRE |
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