Autophagy-Related Protein MAP1LC3C Plays a Crucial Role in Odontogenic Differentiation of Human Dental Pulp Cells
| Autor: | Won Jae Kim, Sam Young Park, Sun Mi Kim, Heui Seung Cho, Ji Yeon Jung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Cellular differentiation ATG8 0206 medical engineering Biomedical Engineering Medicine (miscellaneous) Autophagy-Related Proteins 02 engineering and technology Rats Sprague-Dawley 03 medical and health sciences Downregulation and upregulation Dentin sialophosphoprotein stomatognathic system Gene expression Dentin medicine Animals Humans Cells Cultured Dental Pulp 030304 developmental biology 0303 health sciences Chemistry Autophagy Cell Differentiation 020601 biomedical engineering Cell biology Rats stomatognathic diseases medicine.anatomical_structure Original Article MAP1LC3B Microtubule-Associated Proteins |
| Zdroj: | Tissue Eng Regen Med |
| Popis: | BACKGROUND: Autophagy plays important roles in odontogenic differentiation of dental pulp cells (DPCs) in the developmental stage of tooth bud. Few studies have reported the role of autophagy during reparative dentin formation process. The objective of this study was to discover gene expression pattern correlated to autophagy and their role during odontogenic differentiation process in DPCs. METHODS: After tooth cavities were prepared on the mesial surface of lower first molar crown of rats. Odontogenic differentiation and reparative dentin formation were assessed based on detection of morphology change with hematoxylin and eosin staining. RESULTS: After tooth cavities were prepared on the mesial surface of lower first molar crown of rats, odontogenic differentiation and reparative dentin formation were assessed based on detection of morphology change with hematoxylin and eosin staining and dentin sialophosphoprotein (DSPP), whereas autophagy inhibitor 3-methyladenine (3MA) reversed. Results of quantitative polymerized chain reaction array of autophagosome formation related genes revealed that GABARAPL2 was prominently upregulated while expression of other ATG8 family members were moderately increased after tooth cavity preparation. In addition, human DPCs incubated in differentiation medium predominantly upregulated MAP1LC3C, which selectively decreased by 3MA but not by autophagy enhancer trehalose. Knock-down of MAP1LC3C using shRNA resulted in strong downregulation of dentin matrix protein 1 and DSPP as well-known odontogenic marker compared to knock-down of MAP1LC3B during odontogenic differentiation process of human DPCs. CONCLUSION: Our results suggest that MAP1LC3C plays a crucial role in odontogenic differentiation of human DPCs via regulating autophagic flux. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13770-020-00310-3) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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