The Neonatal FcR-Mediated Presentation of Immune-Complexed Antigen Is Associated with Endosomal and Phagosomal pH and Antigen Stability in Macrophages and Dendritic Cells
| Autor: | Xindong Liu, David M. Mosser, Senthilkumar Palaniyandi, Derry C. Roopenian, Li Lu, Rongyu Zeng, Lian Yong Gao, Xiaoping Zhu, Ziyan Yang |
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| Rok vydání: | 2011 |
| Předmět: |
Ovalbumin
T-Lymphocytes Blotting Western Immunology Antigen presentation Mice Transgenic chemical and pharmacologic phenomena Endosomes Receptors Fc Immunoglobulin G Mice Cross-Priming Immune system Antigen Phagosomes MHC class I Animals Immunology and Allergy Antigens Cells Cultured Cell Proliferation Phagosome Mice Knockout Antigen Presentation biology Macrophages Histocompatibility Antigens Class I Dendritic Cells Hydrogen-Ion Concentration Flow Cytometry Molecular biology Endocytosis Immune complex Mice Inbred C57BL biology.protein Female CD8 Protein Binding |
| Zdroj: | The Journal of Immunology. 186:4674-4686 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | The FcγRs found on macrophages (Mϕs) and dendritic cells (DCs) efficiently facilitate the presentation or cross-presentation of immune-complexed Ags to T cells. We found that the MHC class I-related neonatal FcR for IgG (FcRn) in both Mϕs and DCs failed to have a strong effect on the cross-presentation of immune complex (IC) OVA Ag to CD8+ T cells. Interestingly, endosomal FcRn enhanced the presentation of the monomeric OVA-IC to CD4+ T cells robustly, whereas FcRn in phagosomes exerted distinctive effects on Ag presentation between Mϕs and DCs. The presentation of phagocytosed OVA-ICs to CD4+ T cells was considerably enhanced on wild-type versus FcRn-deficient Mϕs, but was not affected in FcRn-deficient DCs. This functional discrepancy was associated with the dependence of IgG–FcRn binding in an acidic pH. Following phagocytosis, the phagosomal pH dropped rapidly to |
| Databáze: | OpenAIRE |
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