Induction of endoplasmic reticulum stress in retinal pericytes by glucose deprivation
Autor: | Kengo Ikesugi, Christian J. Madson, Peter F. Kador, Michael L. Mulhern, Toshimichi Shinohara, Ken Ichi Hosoya, Tetsuya Terasaki |
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Rok vydání: | 2006 |
Předmět: |
medicine.medical_specialty
Cell Survival Blotting Western Hypoglycemia Biology Endoplasmic Reticulum Cellular and Molecular Neuroscience chemistry.chemical_compound Internal medicine Diabetes mellitus medicine In Situ Nick-End Labeling Animals Caspase 12 Cells Cultured Heat-Shock Proteins Endoplasmic reticulum fungi Retinal Vessels Retinal Diabetic retinopathy medicine.disease Activating Transcription Factor 4 Sensory Systems Rats Ophthalmology Oxidative Stress Endocrinology Glucose chemistry Apoptosis Hyperglycemia Unfolded protein response Pericytes Transcription Factor CHOP Retinopathy Molecular Chaperones |
Zdroj: | Current eye research. 31(11) |
ISSN: | 0271-3683 |
Popis: | Diabetic retinopathy is one of the major microvascular complications associated with diabetes mellitus, and the selective degeneration of retinal capillary pericytes is considered to be a hallmark of early retinopathy. Because glucose fluctuations commonly occur in diabetes, we hypothesized that these fluctuations will increase the endoplasmic reticulum (ER) stress and induce the unfolded protein response (UPR) in retinal pericytes. To study whether ER stress and the UPR can be induced in retinal pericytes, rat retinal capillary pericytes were cultured in different concentrations of glucose. Hypoglycemia but not hyperglycemia was found to activate UPR-specific enzymes in pericytes. Strong UPR activation leading to apoptosis was also observed when pericytes were cultured in glucose concentrations that were reduced from high to low or no glucose. These results indicate that induction of UPR is related not only to absolute concentrations but also to a shifting from higher to lower concentrations of glucose. |
Databáze: | OpenAIRE |
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