Antibody‐based cell‐surface proteome profiling of metastatic breast cancer primary explants and cell lines
| Autor: | Ernest M. Meyer, Albert D. Donnenberg, Jayce Jieming Zhang, Vera S. Donnenberg, Haihui Lu, Christian T. Carson, Erika Moravcikova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Histology Proteome Cell Breast Neoplasms Biology Antibodies Pathology and Forensic Medicine 03 medical and health sciences Breast cancer Cell Line Tumor Biomarkers Tumor medicine Humans Neoplastic transformation Clonogenic assay Cell Membrane Mesenchymal stem cell Epithelial Cells Mesenchymal Stem Cells Cell Biology Flow Cytometry medicine.disease Metastatic breast cancer Up-Regulation Gene Expression Regulation Neoplastic 030104 developmental biology medicine.anatomical_structure A549 Cells MCF-7 Cells Cancer research Female K562 Cells Cytometry |
| Zdroj: | Cytometry Part A. 93:448-457 |
| ISSN: | 1552-4930 1552-4922 |
| Popis: | Flow cytometric cell surface proteomics provides a new and powerful tool to determine changes accompanying neoplastic transformation and invasion, providing clues to essential interactions with the microenvironment as well as leads for potential therapeutic targets. One of the most important advantages of flow cytometric cell surface proteomics is that it can be performed on living cells that can be sorted for further characterization and functional studies. Here, we document the surface proteome of clonogenic metastatic breast cancer (MBrCa) explants, which was strikingly similar to that of normal mesenchymal stromal cells (P = 0.017, associated with Pearson correlation coefficient) and transformed mammary epithelial cells (P = 0.022). Markers specifically upregulated on MBrCa included CD200 (Ox2), CD51/CD61 (Integrin α5/β3), CD26 (dipeptidyl peptidase-4), CD165 (c-Cbl), and CD54 (ICAM-1). Proteins progressively upregulated in a model of neoplastic transformation and invasion included CD26, CD63 (LAMP3), CD105 (Endoglin), CD107a (LAMP1), CD108 (Semaphorin 7A), CD109 (Integrin β4), CD151 (Raph blood group), and disialoganglioside G2. The proteome of the commonly used cell lines MDA-MB-231, MCF7, and BT-474 were uncorrelated with that of MBrCa (P = 1.0, 1.0, 0.9, respectively). The comparison has demonstrated the mesenchymal nature of clonogenic cells isolated by short-term culture of metastatic breast cancer, provided several leads for biomarkers and potential targets for anti-invasive therapy, including CD200, and highlighted the limitations of breast cancer cell lines for representing the cell surface biology of breast cancer. © 2017 International Society for Advancement of Cytometry. |
| Databáze: | OpenAIRE |
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