Biosynthesis of the Diterpene cis-Abienol in Cell-Free Extracts of Tobacco Trichomes
| Autor: | George J. Wagner, Zhenhua Guo, R. F. Severson |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Oxygenase
Geranylgeranyl pyrophosphate Nicotiana tabacum Biophysics Naphthols Tritium Biochemistry Labdane chemistry.chemical_compound Polyisoprenyl Phosphates Biosynthesis Tobacco Magnesium Molecular Biology Chromatography High Pressure Liquid Cell-Free System biology Epidermis (botany) Plant Extracts Hydrogen-Ion Concentration biology.organism_classification Kinetics Plants Toxic chemistry Diterpenes Diterpene Cyclase activity |
| Zdroj: | Archives of Biochemistry and Biophysics. 308:103-108 |
| ISSN: | 0003-9861 |
| Popis: | Biosynthesis of the hydroxylated labdane diterpene, cis-abienol, 9 alpha H-[(12Z)-labda-12,14-dien-8 alpha-ol] from geranylgeranyl pyrophosphate was observed in cell-free extracts prepared from trichome-bearing tissues of the experimental tobacco, Nicotiana tabacum L. c.v. T.I. 1068. Synthetic activity was observed with extracts of trichome glands, but not subepidermal tissue or epidermis from which glands had been removed. The activity was soluble, Mg2+ stimulated, had a pH optimum near 7.0, and was unaffected by conditions which inhibit cytochrome P450 oxygenase. These results indicate the occurrence in trichome glands of T.I. 1068, a cyclase activity which yields the cyclized hydroxylated labdanoid cis-abienol directly. |
| Databáze: | OpenAIRE |
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