A novel bicomponent hemolysin from Bacillus cereus
Autor: | J D MacMillan, D J Beecher |
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Rok vydání: | 1990 |
Předmět: |
Macromolecular Substances
Bacterial Toxins Immunoblotting Immunology Bacillus cereus Hemolytic Plaque Technique Enterotoxin Biology Hemolysin Proteins Microbiology Bacterial Proteins Species Specificity medicine Gel electrophoresis Antigens Bacterial Chromatography Isoelectric focusing Antibodies Monoclonal Fast protein liquid chromatography Hemolysin medicine.disease biology.organism_classification Molecular biology Hemolysis Infectious Diseases Parasitology Research Article |
Zdroj: | Infection and Immunity. 58:2220-2227 |
ISSN: | 1098-5522 0019-9567 |
Popis: | A procedure combining isoelectric focusing (Sephadex IEF) and fast protein liquid chromatography (Superose 12; Mono-Q) removed hemolytic activity (presumably a contaminant) from partially purified preparations of the multicomponent diarrheal enterotoxin produced by Bacillus cereus. However, when the separated fractions were recombined, hemolytic activity was restored, suggesting that hemolysis is a property of the enterotoxin components. Combined fractions exhibited a unique ring pattern in gel diffusion assays in blood agar. During diffusion of the hemolysin from an agar well, the erythrocytes closest to the well were not lysed initially. After diffusion, hemolysis was observed as a sharp ring beginning several millimeters away from the edge of the well. With time the cells closer to the well were also lysed. This novel hemolysin consists of a protein (component B) which binds to or alters cells, allowing subsequent lysis by a second protein (component L). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, and Western blot analysis showed that hemolysin BL has properties similar to those described previously for the enterotoxin and that both components are distinct from cereolysin and cereolysin AB. |
Databáze: | OpenAIRE |
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