For staining of ALK protein, the novel D5F3 antibody demonstrates superior overall performance in terms of intensity and extent of staining in comparison to the currently used ALK1 antibody
| Autor: | David J. Zahavi, Raluca Yonescu, Shahaboddin Dolatkhah, Bernardo F.P. Ricardo, Maria Del Carmen Rodriguez, Yi Ning, Neda Rezaee, Justin A. Bishop, Rajni Sharma, George J. Netto, Diana Taheri, Abdelrazak Meliti |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Pathology medicine.medical_specialty Lung Neoplasms medicine.drug_class Biology Monoclonal antibody Antibodies Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Carcinoma Non-Small-Cell Lung medicine Anaplastic lymphoma kinase Humans Anaplastic Lymphoma Kinase Molecular Biology In Situ Hybridization Fluorescence Gene Rearrangement medicine.diagnostic_test ALK Gene Rearrangement Receptor Protein-Tyrosine Kinases Cell Biology General Medicine Middle Aged Molecular biology Immunohistochemistry Staining 030104 developmental biology 030220 oncology & carcinogenesis Monoclonal biology.protein Female Antibody Gene Fusion Fluorescence in situ hybridization |
| Zdroj: | Virchows Archiv : an international journal of pathology. 469(3) |
| ISSN: | 1432-2307 |
| Popis: | Inflammatory myofibroblastic tumor (IMT) is a rare neoplasm. Approximately 50 % of IMTs show an anaplastic lymphoma kinase (ALK) gene fusion resulting in ALK overexpression on immunohistochemistry (IHC). A novel anti-ALK monoclonal antibody (D5F3) has been suggested to be of superior sensitivity to the ALK1 antibody which is currently used. We compared the performance of D5F3 in detecting ALK protein expression in IMTs from various anatomic sites compared to the currently utilized ALK1. We selected 25 IMTs from our surgical pathology files (2005-2015). The novel rabbit monoclonal anti-human CD246 (clone D5F3) and the currently used mouse monoclonal anti-human CD246 (clone ALK1) were used for immunohistochemical staining (IHC) in an automated slide stainer. The percentage of immunoreactive tumor cells (0,5 %, 5-50 %,50 %) and cytoplasmic staining intensity (graded 0-3) were assessed and compared between the two antibodies. Fluorescence in situ hybridization (FISH) studies for ALK gene rearrangement were performed on 11 tumors. D5F3 antibody stained 76 % and ALK1 antibody stained 72 % of IMTs (p = 0.747). Compared to staining with ALK1, D5F3 stained a higher proportion of cases extensively (50 % cells) (76 vs. 28 %, p 0.001) and with high intensity (grade 3 76 % vs 0; p 0.001). FISH and IHC findings (for both antibodies) were concordant in 9/10 (90 %) IMTs, in which results were informative. The novel anti-ALK rabbit monoclonal antibody (D5F3 clone) demonstrates superior overall performance in term of intensity and extent of staining of ALK protein in IMT. We found IHC staining with both antibody clones to correlate equally well with FISH results for detection of ALK rearrangement. |
| Databáze: | OpenAIRE |
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