The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production
| Autor: | Huan-Wei Huang, Lin Li, Rong-Nan Lin, Tao Cai, She Chen, Hao-Ran Zhou, Xin-Jian He, Jian-Kang Zhu |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Small interfering RNA Arabidopsis Chromatin silencing Plant Science 01 natural sciences 03 medical and health sciences Genetics Gene silencing RNA Small Interfering RNA-Directed DNA Methylation biology Arabidopsis Proteins RNA Cell Biology DNA-Directed RNA Polymerases DNA Methylation biology.organism_classification Cell biology Repressor Proteins 030104 developmental biology DNA methylation 010606 plant biology & botany Genetic screen Transcription Factors |
| Zdroj: | The Plant journal : for cell and molecular biologyREFERENCES. 103(4) |
| ISSN: | 1365-313X |
| Popis: | Small interfering RNAs (siRNAs) are responsible for establishing and maintaining DNA methylation through the RNA-directed DNA methylation (RdDM) pathway in plants. Although siRNA biogenesis is well known, it is relatively unclear about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. We demonstrated that NOT1 is required for Pol IV-dependent siRNA accumulation and DNA methylation at a subset of RdDM target genomic loci. Furthermore, we revealed that NOT1 is a constituent of a multi-subunit CCR4-NOT deadenylase complex by immunoprecipitation combined with mass spectrometry and demonstrated that the CCR4-NOT components can function as a whole to mediate chromatin silencing. Therefore, our work establishes that the CCR4-NOT complex regulates the biogenesis of Pol IV-dependent siRNAs, and hence facilitates DNA methylation and transcriptional silencing in Arabidopsis. |
| Databáze: | OpenAIRE |
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