Rainbow Trout IgM+ B Cells Preferentially Respond to Thymus-Independent Antigens but Are Activated by CD40L
| Autor: | Patricia Díaz-Rosales, Pedro Perdiguero, Carolina Tafalla, Aitor G. Granja, Alba Martín-Martín, Irene Soleto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fish Proteins lcsh:Immunologic diseases. Allergy IgM Immunology CD40 Ligand Thymus Gland Aquatic Science 03 medical and health sciences 0302 clinical medicine Antigen Immunology and Allergy Animals Environmental Chemistry CD40L Antigens Receptor Original Research 030304 developmental biology teleost fish B-Lymphocytes 0303 health sciences B cells CD40 biology Germinal center RNA 04 agricultural and veterinary sciences General Medicine extrafollicular responses 3. Good health Cell biology 030104 developmental biology Immunoglobulin M Immunoglobulin class switching Oncorhynchus mykiss 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Rainbow trout lcsh:RC581-607 030215 immunology |
| Zdroj: | Frontiers in Immunology, Vol 10 (2019) Frontiers in Immunology |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2019.02902/full |
| Popis: | In the absence of class switch recombination and germinal centers, the mechanisms through which B cells from teleost fish mount extrafollicular immunoglobulin M (IgM) memory responses remains mostly unexplored. In this report, we demonstrate that teleost IgM+ B cells respond to CD40L, a thymus-dependent activation signal, similarly to mammalian B2 cells. However, when stimulated with different types of antigens, fish IgM+ B cells only reach a general activation state in response to antigens cataloged as thymus-independent 1 (TI-1) in mammals, as established through both functional assays and RNA sequencing. Interestingly, fish IgM+ B cells remained completely unresponsive to TI-2 antigens, suggesting that the engagement of innate receptors provided by TI-1 antigens is required for the activation of teleost B cells. Finally, a synergy between CD40L and TI-1 antigens was also demonstrated, further supporting that there is no clear dichotomy between thymus-dependent and TI responses in teleost fish. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|