Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens
| Autor: | Zou JianMin, Tu YunJie, Sheng ZhongWei, Shan YanJu, Zhang Ming, Xiao Qin, Shu JingTing, Ji GaiGe |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene Expression Muscle Proteins lcsh:Medicine Biochemistry Poultry Transcriptome 0302 clinical medicine Myofibrils Animal Cells PPARGC1A Gene Gene expression Medicine and Health Sciences Myocyte Gamefowl Gene Regulatory Networks lcsh:Science Musculoskeletal System Oligonucleotide Array Sequence Analysis Regulation of gene expression Multidisciplinary Muscles Cell biology Partridges medicine.anatomical_structure 030220 oncology & carcinogenesis Vertebrates PPARGC1A Anatomy Cellular Types Glycolysis Oxidation-Reduction Research Article Biology Muscle Fibers Birds 03 medical and health sciences Genetics medicine Animals Gene Regulation Muscle Skeletal Gene Expression Profiling lcsh:R Organisms Biology and Life Sciences Proteins Computational Biology Reproducibility of Results Skeletal muscle Soleus Muscles Cell Biology Skeletal Muscle Fibers Gene expression profiling Gene Ontology 030104 developmental biology Skeletal Muscles Gene Expression Regulation Fowl Amniotes lcsh:Q Chickens |
| Zdroj: | PLoS ONE, Vol 12, Iss 8, p e0183118 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0183118 |
| Popis: | Oxidative and glycolytic myofibers have different structures and metabolic characteristics and their ratios are important in determining poultry meat quality. However, the molecular mechanisms underlying their differences are unclear. In this study, global gene expression profiling was conducted in oxidative skeletal muscle (obtained from the soleus, or SOL) and glycolytic skeletal muscle (obtained from the extensor digitorum longus, or EDL) of Chinese Qingyuan partridge chickens, using the Agilent Chicken Gene Expression Chip. A total of 1224 genes with at least 2-fold differences were identified (P < 0.05), of which 654 were upregulated and 570 were downregulated in SOL. GO, KEGG pathway, and co-expressed gene network analyses suggested that PRKAG3, ATP2A2, and PPARGC1A might play important roles in myofiber composition. The function of PPARGC1A gene was further validated. PPARGC1A mRNA expression levels were higher in SOL than in EDL muscles throughout the early postnatal development stages. In myoblast cells, shRNA knockdown of PPARGC1A significantly inhibited some muscle development and transition-related genes, including PPP3CA, MEF2C, and SM (P < 0.01 or P < 0.05), and significantly upregulated the expression of FWM (P < 0.05). Our study demonstrates strong transcriptome differences between oxidative and glycolytic myofibers, and the results suggest that PPARGC1A is a key gene involved in chicken myofiber composition and transition. |
| Databáze: | OpenAIRE |
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