Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents
| Autor: | Gleison A. Casagrande, Lucas Pizzuti, Melyssa Negri, Persiely P. Rosa, Heberton Wender, Claudia C. Gatto, Bianca Boni Dias, Kátia Veronica Tenório, Kelly Mari Pires de Oliveira, Fabiana Gomes da Silva Dantas, Wellinton Jhon Cupozak-Pinheiro, Fernanda de Oliveira Galvão |
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| Rok vydání: | 2020 |
| Předmět: |
Antifungal Agents
Context (language use) Pyrazoline Candida glabrata Microbial Sensitivity Tests 010402 general chemistry Crystallography X-Ray Ligands 01 natural sciences Biochemistry Ames test Inorganic Chemistry Minimum inhibitory concentration chemistry.chemical_compound Coordination Complexes Thiocarbamates Chlorocebus aethiops medicine Animals Humans Vero Cells biology Molecular Structure 010405 organic chemistry Chemistry Spectrum Analysis Broth microdilution Cobalt Antimicrobial biology.organism_classification 0104 chemical sciences Biofilms Pyrazoles Fluconazole medicine.drug HeLa Cells |
| Zdroj: | Journal of inorganic biochemistry. 213 |
| ISSN: | 1873-3344 |
| Popis: | Candida spp. cause invasive fungal infections. One species, Candida glabrata, may present intrinsic resistance to conventional antifungal agents, thereby increasing mortality rates in hospitalized patients. In this context, metal complexes present an alternative for the development of new antifungal drugs owing to their biological and pharmacological activities demonstrated in studies in the last decades. Accordingly, in this study we have synthesized and characterized two new Co(II) complexes with thiocarbamoyl-pyrazoline ligands to assess their antimicrobial, mutagenic, and cytotoxic potential. For antimicrobial activity, the broth microdilution method was performed against ATCC strains of Candida spp. and fluconazole dose-dependent isolates of C. glabrata obtained from urine samples. The Ames test was used to assess mutagenic potential. The reduction method of the MTS reagent (3 [4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium) was performed with HeLa, SiHa, and Vero cells to determine cytotoxicity. Both complexes exhibited fungistatic and fungicidal activity for the yeasts used in the study, demonstrating greater potential for C. glabrata ATCC 2001 and the C. glabrata CG66 isolate with a Minimum Inhibitory Concentration MIC from 3.90 to 7.81 μg mL−1 and fungicidal action from 7.81 to 15.62 μg mL−1. The complexes inhibited and degraded biofilms by up to 90% and did not present mutagenic and cytotoxic potential at the concentrations evaluated for MIC. Thus, the complexes examined herein suggest promising alternatives for the development of new antifungal drugs. |
| Databáze: | OpenAIRE |
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