Diffusion based analysis in a sheath flow microchannel: the sheath flow T-sensor
| Autor: | Melissa S. Hasenbank, Paul Yager, Matthew S. Munson, Kenneth R. Hawkins |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Microchannel
Passivation Rhodamines Wheat Germ Agglutinins Biomedical Engineering Analytical chemistry Bioengineering General Chemistry Microfluidic Analytical Techniques Models Theoretical Biochemistry Molecular physics Wheat germ agglutinin Core (optical fiber) Diffusion chemistry.chemical_compound chemistry Flow (mathematics) Rhodamine B Diffusion (business) Intensity (heat transfer) |
| Zdroj: | Lab on a chip. 5(8) |
| ISSN: | 1473-0197 |
| Popis: | This paper describes a microfluidic channel that allows for diffusion-based analysis of adsorbing species without passivation of the channel surfaces. The sheath flow configuration was used to measure the diffusion coefficient of fluorescently labeled species from their spatial distribution within the microchannel by analyzing the derivative of the intensity profile at the interface between two distinct core fluids. Measurements for both a small molecule (rhodamine B) and an intermediate-sized protein (wheat germ agglutinin) were made, demonstrating the utility of the sheath flow T-sensor. |
| Databáze: | OpenAIRE |
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