Proteome analysis during pod, zygotic and somatic embryo maturation of Theobroma cacao
| Autor: | Mark J. Guiltinan, Tatiana N. Laremore, Edward Q. Kaiser, Siela N. Maximova, Nicolas Niemenak |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Proteomics
Proteome Somatic embryogenesis Zygote Physiology Somatic cell Mabinlin Plant Science Mass Spectrometry Gene Expression Regulation Plant Nanotechnology Storage protein Electrophoresis Gel Two-Dimensional Plant Proteins Genetics chemistry.chemical_classification Cacao biology Gene Expression Regulation Developmental Embryo Cell biology chemistry Seeds Chitinase biology.protein Agronomy and Crop Science Chromatography Liquid |
| Zdroj: | Journal of Plant Physiology. 180:49-60 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2015.02.011 |
| Popis: | Two dimensional electrophoresis and nano-LC–MS were performed in order to identify alterations in protein abundance that correlate with maturation of cacao zygotic and somatic embryos. The cacao pod proteome was also characterized during development. The recently published cacao genome sequence was used to create a predicted proteolytic fragment database. Several hundred protein spots were resolved on each tissue analysis, of which 72 variable spots were subjected to MS analysis, resulting in 49 identifications. The identified proteins represent an array of functional categories, including seed storage, stress response, photosynthesis and translation factors. The seed storage protein was strongly accumulated in cacao zygotic embryos compared to their somatic counterpart. However, sucrose treatment (60 g L −1 ) allows up-regulation of storage protein in SE. A high similarity in the profiles of acidic proteins was observed in mature zygotic and somatic embryos. Differential expression in both tissues was observed in proteins having high p I . Several proteins were detected exclusively in fruit tissues, including a chitinase and a 14-3-3 protein. We also identified a novel cacao protein related to known mabinlin type sweet storage proteins. Moreover, the specific presence of thaumatin-like protein, another sweet protein, was also detected in fruit tissue. We discuss our observed correlations between protein expression profiles, developmental stage and stress responses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect