Live porcine thirty days delayed recovery surgery: Qualitative findings with the hypersonic vitrectomy

Autor: Richard Bonshek, Soon Wai Ch'ng, Joseph Shaw, Paulo E. Stanga, Salvador Pastor-Idoate, Luciane Irion, Alessandro Papayannis
Přispěvatelé: Vavvas, Demetrios G.
Rok vydání: 2018
Předmět:
Medical Doctors
genetic structures
Swine
Health Care Providers
medicine.medical_treatment
lcsh:Medicine
Vitrectomy
chemistry.chemical_compound
0302 clinical medicine
Depigmentation
Medicine and Health Sciences
Postoperative Period
Medical Personnel
lcsh:Science
Mammals
Multidisciplinary
Ophthalmic Procedures
Eukaryota
Professions
Veterinary Surgery
medicine.anatomical_structure
Vertebrates
Optic nerve
Anatomy
medicine.symptom
Research Article
Veterinary Medicine
Pars plana
medicine.medical_specialty
Ocular Anatomy
Enucleation
Surgical and Invasive Medical Procedures
Retina
03 medical and health sciences
Ultrasonic Surgical Procedures
Ocular System
Physicians
Lens
Crystalline
medicine
Animals
Surgeons
Retinal pigment epithelium
business.industry
lcsh:R
Organisms
Biology and Life Sciences
Retinal
eye diseases
Surgery
Health Care
chemistry
Amniotes
People and Places
030221 ophthalmology & optometry
Eyes
Population Groupings
Veterinary Science
lcsh:Q
sense organs
business
Head
030217 neurology & neurosurgery
Zdroj: Ch’ng, S W, Irion, L D, Bonshek, R, Shaw, J, Papayannis, A, Pastor-idoate, S, Stanga, P E & Vavvas, D G (ed.) 2018, ' Live porcine thirty days delayed recovery surgery: Qualitative findings with the hypersonic vitrectomy ', PLoS ONE, vol. 13, no. 6, pp. e0197038 . https://doi.org/10.1371/journal.pone.0197038
PLoS ONE, Vol 13, Iss 6, p e0197038 (2018)
PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0197038
Popis: IntroductionQualitatively assess the possible delayed structural, macroscopic and microscopic changes in the neuro-retina, retinal vasculature, retinal pigment epithelium (RPE) and optic nerve head (ONH) after pars plana vitrectomy (PPV) surgery using a new hypersonic vitrector (HV).Materials and methodsEight live porcine eyes underwent PPV using either the HV or a conventional pneumatic guillotine vitrector (GV). The un-operated fellow eye from each pig was used as an external control. The pigs were post-operatively kept alive for 30 days before termination and enucleation of the eyes. Prior to enucleation, all eyes underwent examination of the lens and indirect ophthalmoscopy. Enucleated eyes were fixed in formalin, examined macroscopically and processed for histological assessment. Microscopic analysis included assessment of neuro-retina, retinal vasculature, RPE and ONH, as well as observation for any morphological intraocular changes. Comparison was made between: (1) treated and untreated areas of the same eye (internal control) (2) different areas within the same eye operated on using different vitrector settings (3) eyes operated on with the HV and GV (4) eyes receiving surgery and the fellow un-operated eye (external control, same pig).ResultsAll lenses had remained clear at 30 days into the postoperative period. On indirect ophthalmoscopy, the retina had remained attached in all eyes with no visible changes to the neuro-retina, retinal vasculature, RPE or ONH. Two eyes showed localized RPE depigmentation secondary to previously documented intraoperative retinal touch. The Morphological changes in the retinal layers above depigmented RPE were no different from retinal change elsewhere. There were mild and similar microscopic changes observed in the neuro-retina, retinal vasculature, RPE or ONH associated with either the HV or GV PPVs. Preliminary histological findings revealed no significant differences between eyes operated on with the HV and those operated on the GV.DiscussionAt 30 days into the postoperative period, there seemed to be similar morphological changes attributable to the use of HV and GV. Therefore, the HV promises to be a new alternative to the currently commercially available GV for PPV.
Databáze: OpenAIRE
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