Immunological and inflammatory profiles during acute and convalescent phases of severe/ critically ill COVID-19 patients

Autor: Lianzhong Li, Qigao Chen, Jiewen Huang, Ying Liang, Bolin Cheng, Jing Zhou, Baodan Yu, Xuechun Peng, Yihao Yang, Yongping Lin
Rok vydání: 2021
Předmět:
0301 basic medicine
CD4-Positive T-Lymphocytes
Male
Chemokine
Myeloid
CD8-Positive T-Lymphocytes
Chemokine CXCL9
Granzymes
0302 clinical medicine
Immunology and Allergy
Cytotoxic T cell
Chemokine CCL2
Membrane Glycoproteins
biology
Middle Aged
Interleukin-10
Killer Cells
Natural
Interleukin 10
Cytokine release syndrome
medicine.anatomical_structure
030220 oncology & carcinogenesis
Acute Disease
Female
Cytokine Release Syndrome
Adult
Critical Illness
Immunology
severe/critical infection
Article
03 medical and health sciences
Immune system
medicine
Humans
Aged
Pharmacology
Inflammation
business.industry
Interleukin-6
Perforin
COVID-19
Convalescence
Dendritic Cells
medicine.disease
ADP-ribosyl Cyclase 1
Granzyme B
Chemokine CXCL10
030104 developmental biology
Granzyme
cytokines /chemokines
biology.protein
immune cell
business
Zdroj: International Immunopharmacology
ISSN: 1878-1705
Popis: BACKGROUND: The 2019 Coronavirus (COVID-19) pandemic poses a huge threat internationally; however, the role of the host immune system in the pathogenesis of COVID-19 is not well understood. METHODS: Cytokine and chemokine levels and characterisation of immune cell subsets from 20 COVID-19 cases after hospital admission (17 critically ill and 3 severe patients) and 16 convalescent patients were determined using a multiplex immunoassay and flow cytometry, respectively. RESULTS: IP-10, MCP-1, MIG, IL-6, and IL-10 levels were significantly higher in acute severe/critically ill patients with COVID-19, whereas were normal in patients who had reached convalescence. CD8 T cells in severe and critically ill COVID-19 patients expressed high levels of cytotoxic granules (granzyme B and perforin)and was hyperactivated as evidenced by the high proportions of CD38. Furthermore, the cytotoxic potential of natural killer (NK) cells, and the frequencies of myeloid dendritic cells and plasmacytoid dendritic cells was reduced in patients with severe and critical COVID-19; however, these dysregulations were found to be restored in convalescent phases. CONCLUSION: Thus, elicitation of the hyperactive cytokine-mediated inflammatory response, dysregulation of CD8 T and NK cells, and deficiency of host myeloid and plasmacytoid DCs, may contribute to COVID-19 pathogenesis and provide insights into potential therapeutic targets and strategies.
Databáze: OpenAIRE
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