Plasma membrane calcium ATPase 4 (PMCA4) co‐ordinates calcium and nitric oxide signaling in regulating murine sperm functional activity
| Autor: | Kun Li, Patricia A. Martin-DeLeon, Kristine E. Olli, Deni S. Galileo |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Nitric Oxide Synthase Type III Physiology ATPase Caveolin 1 Clinical Biochemistry Apoptosis Calcium-Transporting ATPases Nitric Oxide Synthase Type I Nitric Oxide Asthenozoospermia Article Nitric oxide 03 medical and health sciences chemistry.chemical_compound Multienzyme Complexes Peroxynitrous Acid Fluorescence Resonance Energy Transfer medicine Animals Genetic Predisposition to Disease Calcium Signaling Sperm motility Mice Knockout Mice Inbred ICR biology Chemistry Cell Membrane Cell Biology medicine.disease Spermatozoa Sperm Cell biology Mice Inbred C57BL Calcium ATPase Oxidative Stress Fertility Phenotype 030104 developmental biology Sperm Motility biology.protein Plasma membrane Ca2+ ATPase Peroxynitrite |
| Zdroj: | Journal of Cellular Physiology. 233:11-22 |
| ISSN: | 1097-4652 0021-9541 |
| Popis: | Reduced sperm motility (asthenospermia) and resulting infertility arise from deletion of the Plasma Membrane Ca2+ -ATPase 4 (Pmca4) gene which encodes the highly conserved Ca2+ efflux pump, PMCA4. This is the major Ca2+ clearance protein in murine sperm. Since the mechanism underlying asthenospermia in PMCA4's absence or reduced activity is unknown, we investigated if sperm PMCA4 negatively regulates nitric oxide synthases (NOSs) and when absent NO, peroxynitrite, and oxidative stress levels are increased. Using co-immunoprecipitation (Co-IP) and Fluorescence Resonance Energy Transfer (FRET), we show an association of PMCA4 with the NOSs in elevated cytosolic [Ca2+ ] in capacitated and Ca2+ ionophore-treated sperm and with neuronal (nNOS) at basal [Ca2+ ] (ucapacitated sperm). FRET efficiencies for PMCA4-eNOS were 35% and 23% in capacitated and uncapacitated sperm, significantly (p |
| Databáze: | OpenAIRE |
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