CADM1/TSLC1 inactivation by promoter hypermethylation is a frequent event in colorectal carcinogenesis and correlates with late stages of the disease
| Autor: | Jing Li, Shaoheng Zhang, Guanghai Wang, Xiangsheng Fu, Liang Peng, Ai-ming Li, Yu Zhou, Side Liu, Yang Bai, Yali Zhang, Haisheng Yu, Kequan Chen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Tumor suppressor gene Deleted in Colorectal Cancer Colorectal cancer Down-Regulation Immunoglobulins Mouse model of colorectal and intestinal cancer Biology medicine.disease_cause Cell Line Tumor medicine Humans Epigenetics RNA Messenger Promoter Regions Genetic Aged Neoplasm Staging Tumor Suppressor Proteins Cell Adhesion Molecule-1 Cancer DNA Methylation Middle Aged medicine.disease Oncology DNA methylation Cancer research Female Carcinogenesis Colorectal Neoplasms Cell Adhesion Molecules |
| Zdroj: | International journal of cancer. 128(2) |
| ISSN: | 1097-0215 |
| Popis: | Cell adhesion molecule 1 (CADM1/TSLC1), a putative tumor suppressor involving in cell adhesion, proliferation and apoptosis, is frequently inactivated in several carcinomas due to promoter hypermethylation. But alterations of CADM1/TSLC1 in colorectal carcinogenesis and clinical significance have not been elucidated yet. The aim of this study was to determine the role of functional inactivation of CADM1/TSLC1 gene in colorectal tumorigenesis and its potential as a novel epigenetic marker for clinical assessment of patients with colorectal cancer. We measured CADM1/TSLC1 expression levels in 8 colorectal cancer cell lines, 54 primary colorectal carcinomas and their corresponding non-cancerous tissues by reverse transcription polymerase chain reaction, western blot analysis and immunohistochemistry. We analyzed CADM1/TSLC1 promoter methylation status by bisulfite genomic sequencing and the methylation special polymerase chain reaction, and evaluated its correlation with clinicopathological characteristics. All statistical tests were 2-sides. Downregulation of CADM1/TSLC1 expression was observed in 7 of 8 (88%) colorectal cancer cell lines and in 39 of 54 (72%) primary colorectal carcinomas. Hypermethylation of CADM1/TSLC1 promoter region occurred in 6 of 8 (75%) colorectal cancer cell lines and 32 of 54 (59%) primary colorectal carcinomas, and was correlated with advanced colorectal carcinoma. Epigenetic inactivation of CADM1/TSLC1 gene is a frequent alteration in development of colorectal cancer and can be a potential biomarker for molecular staging of patients with colorectal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text