Isolation of Mitogenic Glycophosphopeptides from Cheese Whey Protein Concentrate
| Autor: | Susumu Kumagai, Kunio Yamauchi, Yoshiko Sugita-Konishi, Sung Seob Yun |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
Phosphopeptides Whey protein Size-exclusion chromatography Carbohydrates chemistry.chemical_element Peptide Calcium Biology Lymphocyte Activation Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Mice Adjuvants Immunologic Cheese Animals Molecular Biology chemistry.chemical_classification Mice Inbred BALB C Chromatography Organic Chemistry Glycopeptides Biological activity General Medicine Oligosaccharide Carbohydrate Milk Proteins Glycopeptide Whey Proteins chemistry Mitogens Spleen Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 60:429-433 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.60.429 |
| Popis: | We investigated the immunological function of cheese whey protein concentrate (CWPC), which is a by-product of cheese production, using mitogenic activity in murine splenocytes as an index. A fraction isolated by gel filtration and anion exchange chromatography of CWPC showed high mitogenic activity, comparable to the activity of lipopolysaccharide (LPS). The fraction was detected as a single band on SDS-PAGE. It contained calcium, inorganic phosphorus, and carbohydrate, indicating the active component to be a glycophosphopeptide (GPP). Since Pronase digestion of GPP did not reduce its mitogenic activity, carbohydrate rather than peptide may be important in the activity. When applied on an anti-beta-caseinophosphopeptide (beta-CPP) antibody affinity column, the GPP was separated into two components, one with affinity to beta-CPP and the other without such affinity. Both the components contained N-linked oligosaccharide chains and had the mitogenic activity. These results demonstrate that cheese whey contains a GPP having strong mitogenic activity. |
| Databáze: | OpenAIRE |
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