Miniaturized bioaffinity assessment coupled to mass spectrometry for guided purification of bioactives from toad and cone snail
| Autor: | Sybren S. Wijmenga, Ruud L. E. G. Aspers, Jenny I. Halff, René van Elk, Reka A. Otvos, Andreas W. Ehlers, Richard J. Lewis, Ferry Heus, Wilfried M. A. Niessen, Sébastien Dutertre, Jeroen Kool, August B. Smit |
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| Přispěvatelé: | Vrije Universiteit Amsterdam [Amsterdam] (VU), Radboud university [Nijmegen], Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institute for Molecular Bioscience, University of Queensland [Brisbane], BioAnalytical Chemistry, Molecular and Cellular Neurobiology, Organic Chemistry, Neuroscience Campus Amsterdam - Brain Mechanisms in Health & Disease, AIMMS, Amsterdam Global Change Institute |
| Rok vydání: | 2014 |
| Předmět: |
Conus textile
Venom Toad Bioinformatics Mass spectrometry complex mixtures Article General Biochemistry Genetics and Molecular Biology Cone snail 03 medical and health sciences Acetylcholine binding 0302 clinical medicine Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP) biology.animal nano-liquid chromatography-mass spectrometry (nano-LC-MS) SDG 14 - Life Below Water [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] lcsh:QH301-705.5 GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) 030304 developmental biology 0303 health sciences Chromatography on-line microfluidics Bufo alvarius Bufo marinus General Immunology and Microbiology biology biology.organism_classification 3. Good health Nicotinic acetylcholine receptor lcsh:Biology (General) Proteome sense organs Biophysical Chemistry General Agricultural and Biological Sciences 030217 neurology & neurosurgery |
| Zdroj: | Biology Biology, MDPI 2014, 3 (1), pp.139-156. ⟨10.3390/biology3010139⟩ Biology, 3, 1, pp. 139-156 Heus, F A H, Otvos, R A, Aspers, R L E G, van Elk, R, Halff, J I, Ehlers, A W, Duterte, S, Lewis, R J, Wijmenga, S, Smit, A B, Niessen, W M A & Kool, J 2014, ' Miniaturized bioactivity assessment coupled to mass spectrometry for guided purification of bioactives from toad and cone snail ', Biology, vol. 3, pp. 139-156 . https://doi.org/10.3390/biology3010139 Biology, 3, 139-156. Multidisciplinary Digital Publishing Institute (MDPI) Biology; Volume 3; Issue 1; Pages: 139-156 Biology, 3, 139-156 Biology, Vol 3, Iss 1, Pp 139-156 (2014) |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology3010139 |
| Popis: | A nano-flow high-resolution screening platform, featuring a parallel chip-based microfluidic bioassay and mass spectrometry coupled to nano-liquid chromatography, was applied to screen animal venoms for nicotinic acetylcholine receptor like (nAChR) affinity by using the acetylcholine binding protein, a mimic of the nAChR. The potential of this microfluidic platform is demonstrated by profiling the Conus textile venom proteome, consisting of over 1, 000 peptides. Within one analysis ( |
| Databáze: | OpenAIRE |
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